10 Questions
What is a working example of in situ adsorption of a product during the fermentation process?
Reverse micelles
Which technique is limited by the toxicity of the solvent to the producing cells when integrating extraction with fermentation for in situ recovery?
Extraction using an interface like a membrane
What has the potential to reduce the solvent toxicity issue when integrating extraction in organic solvents with fermentation for in situ recovery?
Extraction using an interface like a membrane
Which technique has shown potential to solve the solvent toxicity issue in large-scale production but is not yet widely used?
Extraction using an interface like a membrane
What can lead to tightening of a purification protocol by combining clarification, concentration, and purification in one step?
Extraction using an interface like a membrane
Which technology can combine clarification, concentration, and significant purification in one step for protein purification?
Aqueous two-phase systems
What is a significant simplification, especially on a large scale, when directly applying an unclarified sample to a purification method?
Aqueous two-phase systems
What approach aims to decrease the number of processing steps by introducing specificity into techniques for primary separation?
Affinity interactions
What technique can be used for protein purification and combines clarification, concentration, and significant purification in one step?
Expanded bed adsorption
What integration technique combines various separation stages into one unit operation, leading to a tightening of a purification protocol?
Affinity interactions
Test your knowledge on phase separation and partitioning in aqueous two phase systems. Explore the factors affecting the distribution of components between the phases and common observations related to small molecules, particulate matter, and macromolecules.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
