Aqueous Two Phase Systems: Phase Separation and Partitioning Quiz
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Questions and Answers

What is a working example of in situ adsorption of a product during the fermentation process?

  • Centrifugation
  • Extraction using organic solvents
  • Reverse micelles (correct)
  • Partitioning of components
  • Which technique is limited by the toxicity of the solvent to the producing cells when integrating extraction with fermentation for in situ recovery?

  • Partitioning of components
  • Extraction using an interface like a membrane (correct)
  • Centrifugation
  • Reverse micelles
  • What has the potential to reduce the solvent toxicity issue when integrating extraction in organic solvents with fermentation for in situ recovery?

  • Partitioning of components
  • Centrifugation
  • Extraction using an interface like a membrane (correct)
  • Reverse micelles
  • Which technique has shown potential to solve the solvent toxicity issue in large-scale production but is not yet widely used?

    <p>Extraction using an interface like a membrane</p> Signup and view all the answers

    What can lead to tightening of a purification protocol by combining clarification, concentration, and purification in one step?

    <p>Extraction using an interface like a membrane</p> Signup and view all the answers

    Which technology can combine clarification, concentration, and significant purification in one step for protein purification?

    <p>Aqueous two-phase systems</p> Signup and view all the answers

    What is a significant simplification, especially on a large scale, when directly applying an unclarified sample to a purification method?

    <p>Aqueous two-phase systems</p> Signup and view all the answers

    What approach aims to decrease the number of processing steps by introducing specificity into techniques for primary separation?

    <p>Affinity interactions</p> Signup and view all the answers

    What technique can be used for protein purification and combines clarification, concentration, and significant purification in one step?

    <p>Expanded bed adsorption</p> Signup and view all the answers

    What integration technique combines various separation stages into one unit operation, leading to a tightening of a purification protocol?

    <p>Affinity interactions</p> Signup and view all the answers

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