Podcast
Questions and Answers
What is a working example of in situ adsorption of a product during the fermentation process?
What is a working example of in situ adsorption of a product during the fermentation process?
- Centrifugation
- Extraction using organic solvents
- Reverse micelles (correct)
- Partitioning of components
Which technique is limited by the toxicity of the solvent to the producing cells when integrating extraction with fermentation for in situ recovery?
Which technique is limited by the toxicity of the solvent to the producing cells when integrating extraction with fermentation for in situ recovery?
- Partitioning of components
- Extraction using an interface like a membrane (correct)
- Centrifugation
- Reverse micelles
What has the potential to reduce the solvent toxicity issue when integrating extraction in organic solvents with fermentation for in situ recovery?
What has the potential to reduce the solvent toxicity issue when integrating extraction in organic solvents with fermentation for in situ recovery?
- Partitioning of components
- Centrifugation
- Extraction using an interface like a membrane (correct)
- Reverse micelles
Which technique has shown potential to solve the solvent toxicity issue in large-scale production but is not yet widely used?
Which technique has shown potential to solve the solvent toxicity issue in large-scale production but is not yet widely used?
What can lead to tightening of a purification protocol by combining clarification, concentration, and purification in one step?
What can lead to tightening of a purification protocol by combining clarification, concentration, and purification in one step?
Which technology can combine clarification, concentration, and significant purification in one step for protein purification?
Which technology can combine clarification, concentration, and significant purification in one step for protein purification?
What is a significant simplification, especially on a large scale, when directly applying an unclarified sample to a purification method?
What is a significant simplification, especially on a large scale, when directly applying an unclarified sample to a purification method?
What approach aims to decrease the number of processing steps by introducing specificity into techniques for primary separation?
What approach aims to decrease the number of processing steps by introducing specificity into techniques for primary separation?
What technique can be used for protein purification and combines clarification, concentration, and significant purification in one step?
What technique can be used for protein purification and combines clarification, concentration, and significant purification in one step?
What integration technique combines various separation stages into one unit operation, leading to a tightening of a purification protocol?
What integration technique combines various separation stages into one unit operation, leading to a tightening of a purification protocol?
