Podcast
Questions and Answers
What is the purpose of decalcification in tissue preparation?
What is the purpose of decalcification in tissue preparation?
- To facilitate infiltration with paraffin wax
- To remove calcium ions from the tissue (correct)
- To remove excess alcohol from the tissue
- To make the tissue firm for sectioning
What is the ideal thickness of tissue sections for routine histopathological studies?
What is the ideal thickness of tissue sections for routine histopathological studies?
- 10-12 μm
- 15-20 μm
- 5-7 μm (correct)
- 3-5 μm
What is the primary function of xylene in tissue preparation?
What is the primary function of xylene in tissue preparation?
- Decalcification
- Clearing (correct)
- Impregnation
- Dehydration
What is the ideal temperature for incubating culture plates for bacterial colonies?
What is the ideal temperature for incubating culture plates for bacterial colonies?
What is the optimal percentage of ethyl alcohol for dehydrating tissues?
What is the optimal percentage of ethyl alcohol for dehydrating tissues?
What is the purpose of hematoxylin and eosin (H & E) staining?
What is the purpose of hematoxylin and eosin (H & E) staining?
What is the primary focus of the first level of diagnosis?
What is the primary focus of the first level of diagnosis?
What is the ideal sample for diagnostic purposes?
What is the ideal sample for diagnostic purposes?
What is the recommended volume of fixatives to be used for each specimen?
What is the recommended volume of fixatives to be used for each specimen?
What is the purpose of fixation of samples?
What is the purpose of fixation of samples?
What is the recommended fixation time for 10% neutral buffered formalin (NBF)?
What is the recommended fixation time for 10% neutral buffered formalin (NBF)?
What is the name of the fixative that is best for shrimp histology?
What is the name of the fixative that is best for shrimp histology?
What are the important gross external signs among finfishes?
What are the important gross external signs among finfishes?
What is the purpose of archiving facilities in a laboratory?
What is the purpose of archiving facilities in a laboratory?
What is the color of the Gram - bacteria after Gram staining?
What is the color of the Gram - bacteria after Gram staining?
What is the indicator used in the oxidase test?
What is the indicator used in the oxidase test?
What is the product of fermentation of glucose?
What is the product of fermentation of glucose?
What is the purpose of the Voges-Proskauer test?
What is the purpose of the Voges-Proskauer test?
What is the principle of the catalase test?
What is the principle of the catalase test?
What is the purpose of the nitrate reduction test?
What is the purpose of the nitrate reduction test?
What is the principle of the ONPG test?
What is the principle of the ONPG test?
What is the purpose of the Kirby-Bauer disc diffusion method?
What is the purpose of the Kirby-Bauer disc diffusion method?
What is the principle behind indirect immunofluorescence?
What is the principle behind indirect immunofluorescence?
What is the main application of ELISA?
What is the main application of ELISA?
What is the main difference between direct and indirect immunofluorescence?
What is the main difference between direct and indirect immunofluorescence?
What is the purpose of bioassay?
What is the purpose of bioassay?
What is the purpose of dot blot hybridization?
What is the purpose of dot blot hybridization?
What are the major components of PCR?
What are the major components of PCR?