API Klinger Iron Agar (KIA) Bacterial Identification Quiz

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Questions and Answers

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

  • To detect the presence of ferrous sulphate and gas production
  • To observe the formation of cracks in the medium
  • To differentiate based on the fermentation of dextrose, lactose, and H2S production (correct)
  • To identify based on the presence of phenol red and sodium thiosulphate

What is the role of phenol red in KIA?

  • Inhibits the production of hydrogen sulphide
  • Promotes the production of gas bubbles
  • Acts as a pH indicator to show a change in the color of the agar (correct)
  • Enhances the fermentation of lactose

What color does the agar turn when acid is produced during the fermentation of dextrose?

  • Red to yellow (correct)
  • Remains pink
  • Yellow to red
  • Black

What does the fermentation of lactose produce in KIA?

<p>A greater quantity of acid that prevents reoxidation and gives a yellow color to the whole medium (B)</p> Signup and view all the answers

How is the presence of hydrogen sulphide detected in KIA?

<p>Positive results are observable in a black color in the agar medium (D)</p> Signup and view all the answers

What indicates the fermentation of glucose but not lactose in KIA during the first 12 hours of incubation?

<p>The media is yellow due to the dextrose (B)</p> Signup and view all the answers

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

<p>To differentiate based on the fermentation of dextrose, lactose, and H2S production (D)</p> Signup and view all the answers

What is the purpose of the API 10 S kit?

<p>To identify enterobacteriaceae and other Gram-negative rods using miniaturized biochemical tests (B)</p> Signup and view all the answers

What is essential for a quality identification of bacteria using the API 10 S kit?

<p>Use of dilution of pure culture (B)</p> Signup and view all the answers

What is the Kirby-Bauer method commonly used for?

<p>Disk diffusion test for antimicrobial susceptibility (B)</p> Signup and view all the answers

What is the purpose of overlaying with mineral oil in LDC, ODC, H2S, and URE tests?

<p>To create anaerobiosis (A)</p> Signup and view all the answers

What happens after the incubation time in the disk diffusion test?

<p>Results show which antimicrobial could be effective against the specific bacteria specie present in the plate (A)</p> Signup and view all the answers

What is the main purpose of the API 10 S kit?

<p>To identify bacteria using miniaturized biochemical tests (C)</p> Signup and view all the answers

What is the qualitative nature of the Kirby-Bauer method?

<p>It is a qualitative test for antimicrobial susceptibility (C)</p> Signup and view all the answers

What is the purpose of the dilution of pure culture in the API 10 S kit?

<p>For a quality identification of the bacteria (A)</p> Signup and view all the answers

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