15 Questions
What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?
To differentiate based on the fermentation of dextrose, lactose, and H2S production
What is the role of phenol red in KIA?
Acts as a pH indicator to show a change in the color of the agar
What color does the agar turn when acid is produced during the fermentation of dextrose?
Red to yellow
What does the fermentation of lactose produce in KIA?
A greater quantity of acid that prevents reoxidation and gives a yellow color to the whole medium
How is the presence of hydrogen sulphide detected in KIA?
Positive results are observable in a black color in the agar medium
What indicates the fermentation of glucose but not lactose in KIA during the first 12 hours of incubation?
The media is yellow due to the dextrose
What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?
To differentiate based on the fermentation of dextrose, lactose, and H2S production
What is the purpose of the API 10 S kit?
To identify enterobacteriaceae and other Gram-negative rods using miniaturized biochemical tests
What is essential for a quality identification of bacteria using the API 10 S kit?
Use of dilution of pure culture
What is the Kirby-Bauer method commonly used for?
Disk diffusion test for antimicrobial susceptibility
What is the purpose of overlaying with mineral oil in LDC, ODC, H2S, and URE tests?
To create anaerobiosis
What happens after the incubation time in the disk diffusion test?
Results show which antimicrobial could be effective against the specific bacteria specie present in the plate
What is the main purpose of the API 10 S kit?
To identify bacteria using miniaturized biochemical tests
What is the qualitative nature of the Kirby-Bauer method?
It is a qualitative test for antimicrobial susceptibility
What is the purpose of the dilution of pure culture in the API 10 S kit?
For a quality identification of the bacteria
Test your knowledge of biochemical identification of bacteria with this quiz on API Klinger Iron Agar (KIA) and its use in differentiating enteric bacteria based on fermentation and H2S production. Explore the components and pH indicator of KIA while learning about its role in bacterial species identification.
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