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API Klinger Iron Agar (KIA) Bacterial Identification Quiz

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API Klinger Iron Agar (KIA) Bacterial Identification Quiz

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Questions and Answers

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

To detect the presence of ferrous sulphate and gas production

To observe the formation of cracks in the medium

To differentiate based on the fermentation of dextrose, lactose, and H2S production (correct)

To identify based on the presence of phenol red and sodium thiosulphate

What is the role of phenol red in KIA?

Inhibits the production of hydrogen sulphide

Promotes the production of gas bubbles

Acts as a pH indicator to show a change in the color of the agar (correct)

Enhances the fermentation of lactose

What color does the agar turn when acid is produced during the fermentation of dextrose?

Red to yellow (correct)

Remains pink

Yellow to red

Black

What does the fermentation of lactose produce in KIA?

A greater quantity of acid that prevents reoxidation and gives a yellow color to the whole medium Signup and view all the answers

How is the presence of hydrogen sulphide detected in KIA?

Positive results are observable in a black color in the agar medium Signup and view all the answers

What indicates the fermentation of glucose but not lactose in KIA during the first 12 hours of incubation?

The media is yellow due to the dextrose Signup and view all the answers

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

To differentiate based on the fermentation of dextrose, lactose, and H2S production Signup and view all the answers

What is the purpose of the API 10 S kit?

To identify enterobacteriaceae and other Gram-negative rods using miniaturized biochemical tests Signup and view all the answers

What is essential for a quality identification of bacteria using the API 10 S kit?

Use of dilution of pure culture Signup and view all the answers

What is the Kirby-Bauer method commonly used for?

Disk diffusion test for antimicrobial susceptibility Signup and view all the answers

What is the purpose of overlaying with mineral oil in LDC, ODC, H2S, and URE tests?

To create anaerobiosis Signup and view all the answers

What happens after the incubation time in the disk diffusion test?

Results show which antimicrobial could be effective against the specific bacteria specie present in the plate Signup and view all the answers

What is the main purpose of the API 10 S kit?

To identify bacteria using miniaturized biochemical tests Signup and view all the answers

What is the qualitative nature of the Kirby-Bauer method?

It is a qualitative test for antimicrobial susceptibility Signup and view all the answers

What is the purpose of the dilution of pure culture in the API 10 S kit?

For a quality identification of the bacteria Signup and view all the answers

API Klinger Iron Agar (KIA) Bacterial Identification Quiz

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Podcast

Questions and Answers

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

What is the role of phenol red in KIA?

What color does the agar turn when acid is produced during the fermentation of dextrose?

What does the fermentation of lactose produce in KIA?

How is the presence of hydrogen sulphide detected in KIA?

What indicates the fermentation of glucose but not lactose in KIA during the first 12 hours of incubation?

What is the purpose of API Klinger Iron Agar (KIA) in the identification of enteric bacteria?

What is the purpose of the API 10 S kit?

What is essential for a quality identification of bacteria using the API 10 S kit?

What is the Kirby-Bauer method commonly used for?

What is the purpose of overlaying with mineral oil in LDC, ODC, H2S, and URE tests?

What happens after the incubation time in the disk diffusion test?

What is the main purpose of the API 10 S kit?

What is the qualitative nature of the Kirby-Bauer method?

What is the purpose of the dilution of pure culture in the API 10 S kit?

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