Antigen-Antibody Interaction

Antigen-Antibody Interactions

• A potential cause of cross-reaction in Ag-Ab rxn is the similarity in epitopes between different antigens.
• The technique used for the detection and quantitation of antigen and antibody is Immunoprecipitation.

Immunodiagnostic Techniques

• Radioimmunoassay (RIA) is used for the detection and quantitation of antigens and antibodies.
• The purpose of RIA is to measure the concentration of antigens or antibodies in a sample.
• Enzyme-Linked Immunosorbent Assay (ELISA) is used for the detection of antigen-specific antibodies.

Antibody Types

• Monoclonal antibodies are produced by a single clone of cells and are specific to a single epitope.

Antigen-Antibody Interactions

• The major difference between antigen-antibody interaction and an enzyme-substrate reaction is the high specificity and reversibility of antigen-antibody interactions.
• Immunofluorescence is used to visualize and locate antigens in cells or tissues.
• Antigen-antibody interactions involve a specific binding between the antigen and antibody through non-covalent bonds.

Applications of Antigen-Antibody Interactions

• Antigen-antibody interactions can be used for the detection and quantitation of antigens and antibodies in a sample.
• A titer in the context of immunologic assays is the highest dilution of a sample that still gives a positive result.

Serologic Diagnosis

• Antibodies can be used in serologic diagnosis to detect the presence of specific antigens or antibodies in a sample.
• Most serologic tests aim to determine the presence or concentration of specific antibodies in a sample.
• Immunologic techniques in clinical specimens are used to diagnose and monitor diseases, and to detect the presence of specific antigens or antibodies.