Antigen-Antibody Interaction

What is a potential cause of cross reaction in Ag-Ab rxn?

Which technique is used for the detection and quantitation of antigen and antibody?

What is the purpose of radioimmunoassay (RIA)?

Which technique is used for the detection of antigen-specific antibodies?

Which technique is used for the identification and comparison of antigens using immunodiffusion?

What type of antibodies are monoclonal antibodies?

What is the major difference between antigen-antibody interaction and an enzyme-substrate reaction?

What is the purpose of immunofluorescence?

What type of interaction is involved in antigen-antibody reactions?

What can antigen-antibody interactions be used for?

What is a titer in the context of immunologic assays?

How can antibodies be used in serologic diagnosis?

What do most serologic tests aim to determine?

What is the purpose of immunologic techniques in clinical specimens?

Antigen-Antibody Interactions

A potential cause of cross-reaction in Ag-Ab rxn is the similarity in epitopes between different antigens.
The technique used for the detection and quantitation of antigen and antibody is Immunoprecipitation.

Immunodiagnostic Techniques

Radioimmunoassay (RIA) is used for the detection and quantitation of antigens and antibodies.
The purpose of RIA is to measure the concentration of antigens or antibodies in a sample.
Enzyme-Linked Immunosorbent Assay (ELISA) is used for the detection of antigen-specific antibodies.

Antibody Types

Monoclonal antibodies are produced by a single clone of cells and are specific to a single epitope.

Antigen-Antibody Interactions

The major difference between antigen-antibody interaction and an enzyme-substrate reaction is the high specificity and reversibility of antigen-antibody interactions.
Immunofluorescence is used to visualize and locate antigens in cells or tissues.
Antigen-antibody interactions involve a specific binding between the antigen and antibody through non-covalent bonds.

Applications of Antigen-Antibody Interactions

Antigen-antibody interactions can be used for the detection and quantitation of antigens and antibodies in a sample.
A titer in the context of immunologic assays is the highest dilution of a sample that still gives a positive result.

Serologic Diagnosis

Antibodies can be used in serologic diagnosis to detect the presence of specific antigens or antibodies in a sample.
Most serologic tests aim to determine the presence or concentration of specific antibodies in a sample.
Immunologic techniques in clinical specimens are used to diagnose and monitor diseases, and to detect the presence of specific antigens or antibodies.

Test your understanding of the bimolecular association between antigens and antibodies, and the non-covalent interactions involved. Explore the specificity and characteristics of antigen-antibody reactions.