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Questions and Answers
Tungsten filaments lamp used for 200-360 nm
Tungsten filaments lamp used for 200-360 nm
False
Cuvette at spectrophotometer function is hold solid analyte
Cuvette at spectrophotometer function is hold solid analyte
False
Chromatography used in qualitative detection only
Chromatography used in qualitative detection only
False
Partitioning is a separation mechanism in GC and LC
Partitioning is a separation mechanism in GC and LC
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Positive pole at electrophoresis is cathode
Positive pole at electrophoresis is cathode
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One advantage of electrophoresis is very high resolution
One advantage of electrophoresis is very high resolution
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Study Notes
Spectrophotometry
- Tungsten filaments lamps are not used for 200-360 nm wavelength range.
Spectrophotometer Components
- Cuvette holds liquid analyte, not solid analyte.
Chromatography
- Chromatography is used for both qualitative and quantitative detection.
- Partitioning and separation are mechanisms used in both Gas Chromatography (GC) and Liquid Chromatography (LC).
- Chromatography is a group of methods, but it's based on the separation of mixtures, not on the absorption of light by substances.
Applications of Chromatography
- Solid Phase Extraction (SPE) is widely used in environmental analysis, food testing, and forensic toxicology.
High Performance Liquid Chromatography (HPLC)
- HPLC is characterized by high resolution power.
- HPLC provides separation through high pressure created by small particles at the column, not low pressure.
Electrophoresis
- One advantage of electrophoresis is that it has very high resolution.
- The positive pole at electrophoresis is the anode, not the cathode.
- Coloring the sample helps with faster confirmation at gel electrophoresis.
Spectrophotometry Principles
- The proportion of light absorbed depends on the number of molecules that interact with the spectrophotometer.
Chromatography Applications
- Chromatography can be used to separate the components of ink.
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Test your knowledge of analytical chemistry with these multiple choice questions covering topics such as spectroscopy, chromatography, and separation techniques.
