30 Questions
What is the main focus of the Luciferase reporter assay?
The promoter controlling the Luciferase gene
Which of the following is NOT a non-coding regulatory sequence discussed in the text?
Exons
What happens if a regulatory element binds to a gene within a cell?
The gene is transcribed and expressed
In the Luciferase reporter assay, what controls the expression of the luciferase gene?
Proteins
Which of the following sequences enhances gene expression?
Promoter proximal element
What is the purpose of placing the promoter controlling Luciferase ahead of it in the Luciferase reporter assay?
To examine the regulatory element controlling luciferase expression
What is the enzyme responsible for converting D-luciferin into oxyluciferin in fireflies?
Firefly luciferase
In the context of gene activity, what is the function of a reporter gene?
Detects gene expression activity
What role does recombinant DNA technology play in studying gene activity?
Allows investigation of gene activity under specific conditions
How does a luciferase reporter assay help in analyzing DNA regulatory sequences?
Detects gene expression levels
What is the fundamental purpose of studying the activity of a gene using a luciferase reporter assay?
To elucidate gene function under specific conditions
Which component enables scientists to study the role of enzymes in investigating transcriptional regulatory sequences?
'Luciferase Reporter Assay'
What is the function of the Core Promoter?
Produces the basal expression of a gene
In protein-protein interactions, what happens when two proteins are located at the side?
They never interact with each other
Which technique is used to study complex protein-protein interactions?
Yeast two-hybrid system
What is the purpose of Co-immunoprecipitation in studying protein interactions?
Isolates only the protein of interest from a cell sample
What is the function of a DNA library?
Stores genetic information for research purposes
Which type of blotting technique is suitable for detecting proteins?
Western blotting
What is the main difference between a genomic library and a cDNA library?
Genomic libraries contain all genetic material, while cDNA libraries contain only exons.
Why are cDNA libraries expected to differ when created from different cell types?
Each cell type has distinct genes expressed, resulting in unique cDNA libraries.
How are DNA fragments recombined with a plasmid in the process of creating a library?
By using restriction endonucleases and ligase
Why are clones of bacteria used to store specific DNA fragments in a library?
Bacteria act as placeholders for easy retrieval of specific DNA fragments.
What would be the outcome if genomic libraries were created from different cells of the same organism?
The genomic libraries would be identical due to the shared genome across cell types.
What is the purpose of introducing recombinant plasmids into bacteria in the DNA library creation process?
To replicate and store DNA fragments efficiently
What is the function of the activation domain in the yeast two-hybrid system?
Responsible for activation of transcription
Which sequence serves as the binding site for Gal4 in yeast?
UAS
In the yeast two-hybrid system, what happens if the DNA-binding domain and activation domain are not close to each other?
Transcription is inhibited
What is the role of Gal4 in the yeast two-hybrid system?
Activates transcription
What are the two domains that make up the transcription factor controlling UAS in yeast?
BD and AD
Which technology is used to create the special yeast cells with reporter genes controlled by UAS and Gal4 factor?
Recombinant DNA technology
Explore techniques for analyzing DNA regulatory sequences and investigating protein-protein interactions. Learn about the role of enzymes in analyzing transcriptional regulatory sequences.
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