Podcast
Questions and Answers
What is the main focus of the Luciferase reporter assay?
What is the main focus of the Luciferase reporter assay?
- The enhancer sequences
- The TATA box
- The gene of interest
- The promoter controlling the Luciferase gene (correct)
Which of the following is NOT a non-coding regulatory sequence discussed in the text?
Which of the following is NOT a non-coding regulatory sequence discussed in the text?
- TATA box
- Silencers
- Enhancers
- Exons (correct)
What happens if a regulatory element binds to a gene within a cell?
What happens if a regulatory element binds to a gene within a cell?
- The cell undergoes apoptosis
- The gene is transcribed and expressed (correct)
- The gene is silenced
- The gene mutates
In the Luciferase reporter assay, what controls the expression of the luciferase gene?
In the Luciferase reporter assay, what controls the expression of the luciferase gene?
Which of the following sequences enhances gene expression?
Which of the following sequences enhances gene expression?
What is the purpose of placing the promoter controlling Luciferase ahead of it in the Luciferase reporter assay?
What is the purpose of placing the promoter controlling Luciferase ahead of it in the Luciferase reporter assay?
What is the enzyme responsible for converting D-luciferin into oxyluciferin in fireflies?
What is the enzyme responsible for converting D-luciferin into oxyluciferin in fireflies?
In the context of gene activity, what is the function of a reporter gene?
In the context of gene activity, what is the function of a reporter gene?
What role does recombinant DNA technology play in studying gene activity?
What role does recombinant DNA technology play in studying gene activity?
How does a luciferase reporter assay help in analyzing DNA regulatory sequences?
How does a luciferase reporter assay help in analyzing DNA regulatory sequences?
What is the fundamental purpose of studying the activity of a gene using a luciferase reporter assay?
What is the fundamental purpose of studying the activity of a gene using a luciferase reporter assay?
Which component enables scientists to study the role of enzymes in investigating transcriptional regulatory sequences?
Which component enables scientists to study the role of enzymes in investigating transcriptional regulatory sequences?
What is the function of the Core Promoter?
What is the function of the Core Promoter?
In protein-protein interactions, what happens when two proteins are located at the side?
In protein-protein interactions, what happens when two proteins are located at the side?
Which technique is used to study complex protein-protein interactions?
Which technique is used to study complex protein-protein interactions?
What is the purpose of Co-immunoprecipitation in studying protein interactions?
What is the purpose of Co-immunoprecipitation in studying protein interactions?
What is the function of a DNA library?
What is the function of a DNA library?
Which type of blotting technique is suitable for detecting proteins?
Which type of blotting technique is suitable for detecting proteins?
What is the main difference between a genomic library and a cDNA library?
What is the main difference between a genomic library and a cDNA library?
Why are cDNA libraries expected to differ when created from different cell types?
Why are cDNA libraries expected to differ when created from different cell types?
How are DNA fragments recombined with a plasmid in the process of creating a library?
How are DNA fragments recombined with a plasmid in the process of creating a library?
Why are clones of bacteria used to store specific DNA fragments in a library?
Why are clones of bacteria used to store specific DNA fragments in a library?
What would be the outcome if genomic libraries were created from different cells of the same organism?
What would be the outcome if genomic libraries were created from different cells of the same organism?
What is the purpose of introducing recombinant plasmids into bacteria in the DNA library creation process?
What is the purpose of introducing recombinant plasmids into bacteria in the DNA library creation process?
What is the function of the activation domain in the yeast two-hybrid system?
What is the function of the activation domain in the yeast two-hybrid system?
Which sequence serves as the binding site for Gal4 in yeast?
Which sequence serves as the binding site for Gal4 in yeast?
In the yeast two-hybrid system, what happens if the DNA-binding domain and activation domain are not close to each other?
In the yeast two-hybrid system, what happens if the DNA-binding domain and activation domain are not close to each other?
What is the role of Gal4 in the yeast two-hybrid system?
What is the role of Gal4 in the yeast two-hybrid system?
What are the two domains that make up the transcription factor controlling UAS in yeast?
What are the two domains that make up the transcription factor controlling UAS in yeast?
Which technology is used to create the special yeast cells with reporter genes controlled by UAS and Gal4 factor?
Which technology is used to create the special yeast cells with reporter genes controlled by UAS and Gal4 factor?
Flashcards are hidden until you start studying
