Analysis of DNA Regulatory Sequences and Protein-Protein Interaction

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What is the main focus of the Luciferase reporter assay?

The enhancer sequences

The TATA box

The gene of interest

The promoter controlling the Luciferase gene (correct)

Which of the following is NOT a non-coding regulatory sequence discussed in the text?

TATA box

Silencers

Enhancers

Exons (correct)

What happens if a regulatory element binds to a gene within a cell?

The cell undergoes apoptosis

The gene is transcribed and expressed (correct)

The gene is silenced

The gene mutates

In the Luciferase reporter assay, what controls the expression of the luciferase gene?

Proteins Signup and view all the answers

Which of the following sequences enhances gene expression?

Promoter proximal element Signup and view all the answers

What is the purpose of placing the promoter controlling Luciferase ahead of it in the Luciferase reporter assay?

To examine the regulatory element controlling luciferase expression Signup and view all the answers

What is the enzyme responsible for converting D-luciferin into oxyluciferin in fireflies?

Firefly luciferase Signup and view all the answers

In the context of gene activity, what is the function of a reporter gene?

Detects gene expression activity Signup and view all the answers

What role does recombinant DNA technology play in studying gene activity?

Allows investigation of gene activity under specific conditions Signup and view all the answers

How does a luciferase reporter assay help in analyzing DNA regulatory sequences?

Detects gene expression levels Signup and view all the answers

What is the fundamental purpose of studying the activity of a gene using a luciferase reporter assay?

To elucidate gene function under specific conditions Signup and view all the answers

Which component enables scientists to study the role of enzymes in investigating transcriptional regulatory sequences?

'Luciferase Reporter Assay' Signup and view all the answers

What is the function of the Core Promoter?

Produces the basal expression of a gene Signup and view all the answers

In protein-protein interactions, what happens when two proteins are located at the side?

They never interact with each other Signup and view all the answers

Which technique is used to study complex protein-protein interactions?

Yeast two-hybrid system Signup and view all the answers

What is the purpose of Co-immunoprecipitation in studying protein interactions?

Isolates only the protein of interest from a cell sample Signup and view all the answers

What is the function of a DNA library?

Stores genetic information for research purposes Signup and view all the answers

Which type of blotting technique is suitable for detecting proteins?

Western blotting Signup and view all the answers

What is the main difference between a genomic library and a cDNA library?

Genomic libraries contain all genetic material, while cDNA libraries contain only exons. Signup and view all the answers

Why are cDNA libraries expected to differ when created from different cell types?

Each cell type has distinct genes expressed, resulting in unique cDNA libraries. Signup and view all the answers

How are DNA fragments recombined with a plasmid in the process of creating a library?

By using restriction endonucleases and ligase Signup and view all the answers

Why are clones of bacteria used to store specific DNA fragments in a library?

Bacteria act as placeholders for easy retrieval of specific DNA fragments. Signup and view all the answers

What would be the outcome if genomic libraries were created from different cells of the same organism?

The genomic libraries would be identical due to the shared genome across cell types. Signup and view all the answers

What is the purpose of introducing recombinant plasmids into bacteria in the DNA library creation process?

To replicate and store DNA fragments efficiently Signup and view all the answers

What is the function of the activation domain in the yeast two-hybrid system?

Responsible for activation of transcription Signup and view all the answers

Which sequence serves as the binding site for Gal4 in yeast?

UAS Signup and view all the answers

In the yeast two-hybrid system, what happens if the DNA-binding domain and activation domain are not close to each other?

Transcription is inhibited Signup and view all the answers

What is the role of Gal4 in the yeast two-hybrid system?

Activates transcription Signup and view all the answers

What are the two domains that make up the transcription factor controlling UAS in yeast?

BD and AD Signup and view all the answers

Which technology is used to create the special yeast cells with reporter genes controlled by UAS and Gal4 factor?

Recombinant DNA technology Signup and view all the answers