Analysis of DNA Regulatory Sequences and Protein-Protein Interaction
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Questions and Answers

What is the main focus of the Luciferase reporter assay?

  • The enhancer sequences
  • The TATA box
  • The gene of interest
  • The promoter controlling the Luciferase gene (correct)

    • Which of the following is NOT a non-coding regulatory sequence discussed in the text?

  • TATA box
  • Silencers
  • Enhancers
  • Exons (correct)

    • What happens if a regulatory element binds to a gene within a cell?

  • The cell undergoes apoptosis
  • The gene is transcribed and expressed (correct)
  • The gene is silenced
  • The gene mutates

    • In the Luciferase reporter assay, what controls the expression of the luciferase gene?

    <p>Proteins</p> Signup and view all the answers

    Which of the following sequences enhances gene expression?

    <p>Promoter proximal element</p> Signup and view all the answers

    What is the purpose of placing the promoter controlling Luciferase ahead of it in the Luciferase reporter assay?

    <p>To examine the regulatory element controlling luciferase expression</p> Signup and view all the answers

    What is the enzyme responsible for converting D-luciferin into oxyluciferin in fireflies?

    <p>Firefly luciferase</p> Signup and view all the answers

    In the context of gene activity, what is the function of a reporter gene?

    <p>Detects gene expression activity</p> Signup and view all the answers

    What role does recombinant DNA technology play in studying gene activity?

    <p>Allows investigation of gene activity under specific conditions</p> Signup and view all the answers

    How does a luciferase reporter assay help in analyzing DNA regulatory sequences?

    <p>Detects gene expression levels</p> Signup and view all the answers

    What is the fundamental purpose of studying the activity of a gene using a luciferase reporter assay?

    <p>To elucidate gene function under specific conditions</p> Signup and view all the answers

    Which component enables scientists to study the role of enzymes in investigating transcriptional regulatory sequences?

    <p>'Luciferase Reporter Assay'</p> Signup and view all the answers

    What is the function of the Core Promoter?

    <p>Produces the basal expression of a gene</p> Signup and view all the answers

    In protein-protein interactions, what happens when two proteins are located at the side?

    <p>They never interact with each other</p> Signup and view all the answers

    Which technique is used to study complex protein-protein interactions?

    <p>Yeast two-hybrid system</p> Signup and view all the answers

    What is the purpose of Co-immunoprecipitation in studying protein interactions?

    <p>Isolates only the protein of interest from a cell sample</p> Signup and view all the answers

    What is the function of a DNA library?

    <p>Stores genetic information for research purposes</p> Signup and view all the answers

    Which type of blotting technique is suitable for detecting proteins?

    <p>Western blotting</p> Signup and view all the answers

    What is the main difference between a genomic library and a cDNA library?

    <p>Genomic libraries contain all genetic material, while cDNA libraries contain only exons.</p> Signup and view all the answers

    Why are cDNA libraries expected to differ when created from different cell types?

    <p>Each cell type has distinct genes expressed, resulting in unique cDNA libraries.</p> Signup and view all the answers

    How are DNA fragments recombined with a plasmid in the process of creating a library?

    <p>By using restriction endonucleases and ligase</p> Signup and view all the answers

    Why are clones of bacteria used to store specific DNA fragments in a library?

    <p>Bacteria act as placeholders for easy retrieval of specific DNA fragments.</p> Signup and view all the answers

    What would be the outcome if genomic libraries were created from different cells of the same organism?

    <p>The genomic libraries would be identical due to the shared genome across cell types.</p> Signup and view all the answers

    What is the purpose of introducing recombinant plasmids into bacteria in the DNA library creation process?

    <p>To replicate and store DNA fragments efficiently</p> Signup and view all the answers

    What is the function of the activation domain in the yeast two-hybrid system?

    <p>Responsible for activation of transcription</p> Signup and view all the answers

    Which sequence serves as the binding site for Gal4 in yeast?

    <p>UAS</p> Signup and view all the answers

    In the yeast two-hybrid system, what happens if the DNA-binding domain and activation domain are not close to each other?

    <p>Transcription is inhibited</p> Signup and view all the answers

    What is the role of Gal4 in the yeast two-hybrid system?

    <p>Activates transcription</p> Signup and view all the answers

    What are the two domains that make up the transcription factor controlling UAS in yeast?

    <p>BD and AD</p> Signup and view all the answers

    Which technology is used to create the special yeast cells with reporter genes controlled by UAS and Gal4 factor?

    <p>Recombinant DNA technology</p> Signup and view all the answers

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