Amino Acid Isoelectric Point Calculation

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10 Questions

Which type of solution resists change in pH following the addition of an acid or base?

Buffer solution

What is the maximum buffering capacity of a buffer at?

pH equal to pKa

What happens to the pH of a solution when the amounts of HA and A- in a buffer are equal?

pH equals pKa

What is the dissociation constant of the carboxyl group of an amino acid referred to as?

$K_1$

What does each acidic and basic amino acid contain in its side chain?

An ionizable group

Which element is represented by HA in the context of weak acids?

Hydrogen

What is the conjugate base of a weak acid represented by A-?

$HA^-$

In a buffer solution, what happens when a base is added?

$pH$ remains constant

'Salt' or conjugate base A- is the ionized form of what?

$HA$

What defines a buffer's ability to resist changes in pH?

Conjugate acid concentration

Learn how to calculate the isoelectric point (pI) of amino acids, which is the pH at which an amino acid is electrically neutral. Understand the concept of pI as the average of dissociation constants pK1 and pK2 for amino acids with two dissociable hydrogens.

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