10 Questions
What is the primary use of the Henderson-Hasselbalch equation?
To study the dissociation of the carboxyl group of alanine
Which form of alanine predominates at neutral pH?
Form II
At what pH does equal amounts of Forms II and III of alanine exist in solution?
$pH = 9.1$
Which group in alanine is considered a weaker acid compared to the carboxyl group?
-NH3+ group
What does each titratable group of alanine have a pKa numerically equal to?
The pH at which one half of the protons are removed
Which form of alanine exists when the pH equals pK1?
Form I
Which pair of groups can serve as a buffer around pK1?
-COOH/-COO- pair
What is the isoelectric point for alanine?
$pH = 7.0$
How does the dissociation constant of the -NH3+ group compare to that of the -COOH group in alanine?
-NH3+ group has a larger dissociation constant
$K2$ in the text refers to the dissociation constant for which group in alanine?
-NH3+ group
Learn how to calculate the isoelectric point (pI) of an amino acid by averaging the pK values of its dissociable hydrogens. Understand the concept of pI as the pH where an amino acid is electrically neutral.
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