28 Questions
What is the primary use of agar in microbiology?
As a solidifying agent for culture media
What is the temperature range at which agar liquefies?
85°C to 100°C
Which of the following algae is the preferred source of agar?
Gelidium
What is the purpose of controlling the gelation temperature of agar in bacteriological grade agar?
To ensure uniform solidification of culture media
What type of bacteria is commonly grown using LB (Lysogeny Broth) culture media?
Escherichia coli
What is the primary characteristic of a synthetic medium?
It is prepared from only pure chemical substances
Which type of medium is used to isolate a specific bacterial species from a mixed flora?
Selective medium
What is the purpose of adding components like yeast extract or casein hydrolysate to a complex medium?
To provide a nutrient-rich environment for bacterial growth
Which of the following media is an example of an enriched medium?
Blood agar
What is the specific requirement of Streptococcus for its proliferation?
Blood
What is the primary purpose of adjusting the pH to 7.0 in preparing LB broth and LB agar?
To create a favorable environment for bacterial growth
Which of the following bacteria can be grown on Chocolate agar?
Pneumococcus, Haemophilus, Neisseria
What is the main difference between LB broth and LB agar?
The concentration of agar
What type of medium is used to facilitate the growth of motile bacteria?
Semisolid medium
What is the characteristic of a simple medium, such as nutrient broth?
It is commonly used in routine diagnostic laboratories
What is the purpose of adding meat extract to peptone water to create nutrient broth?
To provide additional nutrients for bacterial growth
What is the classification of culture media based on the presence or absence of oxygen?
Aerobic and anaerobic media
What is the primary purpose of adding 1% sugar to a microbial culture medium?
To facilitate the fermentation of glucose and produce acid
Which type of medium is used to grow anaerobic organisms?
Anaerobic medium
What is the characteristic of Salmonella typhi colonies on Wilson and Blair medium?
They are black in color
What is the purpose of using Durham's tube in a sugar medium?
To observe the production of gas
What is the definition of microbial culture?
The method of multiplying microbial organisms in a controlled laboratory environment
What is the purpose of demonstrating the properties of bacteria in culture?
To obtain sufficient growth for antigen preparation
Which culture method is used to provide a uniform surface growth of the bacterium?
Lawn culture
What is the purpose of the streak culture method?
To isolate the bacteria in pure culture
What is the purpose of the pour plate method?
To estimate the viable bacterial count
What is the purpose of the stab culture method?
To provide a pure growth of bacterium for slide agglutination
Which culture method is used for bacteriophage typing?
Culture
Test your knowledge on the properties and characteristics of agar, a crucial solidifying agent used in culture media for microbiological experiments. Learn about its physical properties, sources, and specifications for bacteriological grade agar.
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