Agar Properties in Microbiology
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Questions and Answers

What is the primary use of agar in microbiology?

  • As a pH buffer for bacterial cultures
  • As a solidifying agent for culture media (correct)
  • As a metabolic inhibitor for bacterial growth
  • As a source of nutrients for bacterial growth

    • What is the temperature range at which agar liquefies?

  • 40°C to 50°C
  • 60°C to 70°C
  • 110°C to 120°C
  • 85°C to 100°C (correct)

    • Which of the following algae is the preferred source of agar?

  • Gelidium (correct)
  • Pterocladia
  • Gracilaria
  • Any of the above

    • What is the purpose of controlling the gelation temperature of agar in bacteriological grade agar?

    <p>To ensure uniform solidification of culture media</p> Signup and view all the answers

    What type of bacteria is commonly grown using LB (Lysogeny Broth) culture media?

    <p>Escherichia coli</p> Signup and view all the answers

    What is the primary characteristic of a synthetic medium?

    <p>It is prepared from only pure chemical substances</p> Signup and view all the answers

    Which type of medium is used to isolate a specific bacterial species from a mixed flora?

    <p>Selective medium</p> Signup and view all the answers

    What is the purpose of adding components like yeast extract or casein hydrolysate to a complex medium?

    <p>To provide a nutrient-rich environment for bacterial growth</p> Signup and view all the answers

    Which of the following media is an example of an enriched medium?

    <p>Blood agar</p> Signup and view all the answers

    What is the specific requirement of Streptococcus for its proliferation?

    <p>Blood</p> Signup and view all the answers

    What is the primary purpose of adjusting the pH to 7.0 in preparing LB broth and LB agar?

    <p>To create a favorable environment for bacterial growth</p> Signup and view all the answers

    Which of the following bacteria can be grown on Chocolate agar?

    <p>Pneumococcus, Haemophilus, Neisseria</p> Signup and view all the answers

    What is the main difference between LB broth and LB agar?

    <p>The concentration of agar</p> Signup and view all the answers

    What type of medium is used to facilitate the growth of motile bacteria?

    <p>Semisolid medium</p> Signup and view all the answers

    What is the characteristic of a simple medium, such as nutrient broth?

    <p>It is commonly used in routine diagnostic laboratories</p> Signup and view all the answers

    What is the purpose of adding meat extract to peptone water to create nutrient broth?

    <p>To provide additional nutrients for bacterial growth</p> Signup and view all the answers

    What is the classification of culture media based on the presence or absence of oxygen?

    <p>Aerobic and anaerobic media</p> Signup and view all the answers

    What is the primary purpose of adding 1% sugar to a microbial culture medium?

    <p>To facilitate the fermentation of glucose and produce acid</p> Signup and view all the answers

    Which type of medium is used to grow anaerobic organisms?

    <p>Anaerobic medium</p> Signup and view all the answers

    What is the characteristic of Salmonella typhi colonies on Wilson and Blair medium?

    <p>They are black in color</p> Signup and view all the answers

    What is the purpose of using Durham's tube in a sugar medium?

    <p>To observe the production of gas</p> Signup and view all the answers

    What is the definition of microbial culture?

    <p>The method of multiplying microbial organisms in a controlled laboratory environment</p> Signup and view all the answers

    What is the purpose of demonstrating the properties of bacteria in culture?

    <p>To obtain sufficient growth for antigen preparation</p> Signup and view all the answers

    Which culture method is used to provide a uniform surface growth of the bacterium?

    <p>Lawn culture</p> Signup and view all the answers

    What is the purpose of the streak culture method?

    <p>To isolate the bacteria in pure culture</p> Signup and view all the answers

    What is the purpose of the pour plate method?

    <p>To estimate the viable bacterial count</p> Signup and view all the answers

    What is the purpose of the stab culture method?

    <p>To provide a pure growth of bacterium for slide agglutination</p> Signup and view all the answers

    Which culture method is used for bacteriophage typing?

    <p>Culture</p> Signup and view all the answers

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