Agar Properties in Microbiology

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Questions and Answers

What is the primary use of agar in microbiology?

  • As a pH buffer for bacterial cultures
  • As a solidifying agent for culture media (correct)
  • As a metabolic inhibitor for bacterial growth
  • As a source of nutrients for bacterial growth

What is the temperature range at which agar liquefies?

  • 40°C to 50°C
  • 60°C to 70°C
  • 110°C to 120°C
  • 85°C to 100°C (correct)

Which of the following algae is the preferred source of agar?

  • Gelidium (correct)
  • Pterocladia
  • Gracilaria
  • Any of the above

What is the purpose of controlling the gelation temperature of agar in bacteriological grade agar?

<p>To ensure uniform solidification of culture media (C)</p> Signup and view all the answers

What type of bacteria is commonly grown using LB (Lysogeny Broth) culture media?

<p>Escherichia coli (D)</p> Signup and view all the answers

What is the primary characteristic of a synthetic medium?

<p>It is prepared from only pure chemical substances (A)</p> Signup and view all the answers

Which type of medium is used to isolate a specific bacterial species from a mixed flora?

<p>Selective medium (D)</p> Signup and view all the answers

What is the purpose of adding components like yeast extract or casein hydrolysate to a complex medium?

<p>To provide a nutrient-rich environment for bacterial growth (C)</p> Signup and view all the answers

Which of the following media is an example of an enriched medium?

<p>Blood agar (A)</p> Signup and view all the answers

What is the specific requirement of Streptococcus for its proliferation?

<p>Blood (C)</p> Signup and view all the answers

What is the primary purpose of adjusting the pH to 7.0 in preparing LB broth and LB agar?

<p>To create a favorable environment for bacterial growth (A)</p> Signup and view all the answers

Which of the following bacteria can be grown on Chocolate agar?

<p>Pneumococcus, Haemophilus, Neisseria (A)</p> Signup and view all the answers

What is the main difference between LB broth and LB agar?

<p>The concentration of agar (A)</p> Signup and view all the answers

What type of medium is used to facilitate the growth of motile bacteria?

<p>Semisolid medium (A)</p> Signup and view all the answers

What is the characteristic of a simple medium, such as nutrient broth?

<p>It is commonly used in routine diagnostic laboratories (C)</p> Signup and view all the answers

What is the purpose of adding meat extract to peptone water to create nutrient broth?

<p>To provide additional nutrients for bacterial growth (A)</p> Signup and view all the answers

What is the classification of culture media based on the presence or absence of oxygen?

<p>Aerobic and anaerobic media (A)</p> Signup and view all the answers

What is the primary purpose of adding 1% sugar to a microbial culture medium?

<p>To facilitate the fermentation of glucose and produce acid (B)</p> Signup and view all the answers

Which type of medium is used to grow anaerobic organisms?

<p>Anaerobic medium (A)</p> Signup and view all the answers

What is the characteristic of Salmonella typhi colonies on Wilson and Blair medium?

<p>They are black in color (D)</p> Signup and view all the answers

What is the purpose of using Durham's tube in a sugar medium?

<p>To observe the production of gas (A)</p> Signup and view all the answers

What is the definition of microbial culture?

<p>The method of multiplying microbial organisms in a controlled laboratory environment (C)</p> Signup and view all the answers

What is the purpose of demonstrating the properties of bacteria in culture?

<p>To obtain sufficient growth for antigen preparation (B)</p> Signup and view all the answers

Which culture method is used to provide a uniform surface growth of the bacterium?

<p>Lawn culture (D)</p> Signup and view all the answers

What is the purpose of the streak culture method?

<p>To isolate the bacteria in pure culture (A)</p> Signup and view all the answers

What is the purpose of the pour plate method?

<p>To estimate the viable bacterial count (C)</p> Signup and view all the answers

What is the purpose of the stab culture method?

<p>To provide a pure growth of bacterium for slide agglutination (D)</p> Signup and view all the answers

Which culture method is used for bacteriophage typing?

<p>Culture (A)</p> Signup and view all the answers

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