Aerobic Respiration in Yeast Experiment

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Questions and Answers

What is the independent variable in the investigation of aerobic respiration in yeast?

  • pH level
  • Volume of yeast suspension
  • Concentration of glucose
  • Temperature (correct)

Why is it essential to maintain a constant concentration of dye in the experiment?

  • To allow for a consistent volume of substrate
  • To ensure the yeast cells are not exposed to toxins
  • To avoid interfering with the temperature measurement
  • To keep the respiration rates consistent across tests (correct)

What happens to the rate of aerobic respiration when the temperature exceeds 40°C?

  • It remains constant
  • It increases significantly
  • It fluctuates inconsistently
  • It decreases due to enzyme denaturation (correct)

How is the rate of aerobic respiration measured in this investigation?

<p>By the time taken for the dye to change color (B)</p> Signup and view all the answers

What can be inferred about the relationship between temperature and the kinetic energy of molecules involved in respiration?

<p>Increased temperature raises kinetic energy, facilitating more frequent collisions (A)</p> Signup and view all the answers

What does the discolouration of methylene blue indicate in the experiment involving yeast cells?

<p>It measures the rate of aerobic respiration. (D)</p> Signup and view all the answers

Which method is NOT appropriate for investigating the respiration rate of yeast?

<p>Adding a large quantity of oxygen to the mixture. (C)</p> Signup and view all the answers

What is the role of glucose solution in the respiration experiment with yeast?

<p>It provides a source of energy for yeast respiration. (C)</p> Signup and view all the answers

In the context of the experiment, what happens when the temperature is increased?

<p>The rate of respiration typically increases. (A)</p> Signup and view all the answers

What experimental equipment is NOT directly used to measure respiration rate?

<p>Spectrophotometer (C)</p> Signup and view all the answers

Flashcards

Yeast Respiration

The process by which yeast cells break down glucose to release energy.

Aerobic Respiration

Respiration that requires oxygen.

Methylene Blue

A blue dye used as an indicator to measure respiration rate.

Respiration Rate

How fast an organism is respiring.

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Temperature effect on Respiration Rate

Warmer temperatures generally speed up the rate of respiration.

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Colored Indicator

A substance that changes color to show changes in the environment.

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Yeast Suspension

A mixture of yeast cells in a liquid.

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Glucose Solution

A solution containing glucose, used as a food source for yeast.

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Discoloration Time

The time taken for the methylene blue to lose its color.

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Independent Variable

The variable deliberately changed in an experiment to observe its effect on another variable.

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Dependent Variable

The variable measured and observed in relation to the independent variable.

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Aerobic Respiration

The process by which organisms use oxygen to release energy from glucose.

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Optimum Temperature

The temperature at which a biochemical process, such as respiration, occurs fastest/most efficiently.

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Enzyme Denaturation

The change in shape of an enzyme's active site, preventing it from binding to its substrate and thus reducing its activity.

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Methylene Blue

A dye used as an indicator in experiments measuring the rate of aerobic respiration; its colour changes in the presence of oxygen.

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Yeast Respiration Rate

Measure of how quickly yeast uses oxygen to create energy.

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Controlling Variables

Keeping other factors constant to ensure the only the independent variable changes the dependent variable.

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Temperature Effect on Respiration

The rate of aerobic respiration increases with temperature up to a certain point (optimum temperature) and then decreases because of enzyme denaturation.

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