Advantages and Limitations of Yeast Two-Hybrid (Y2H) Screen

One advantage of a Y2H screen is that it is a relatively fast and easy way to screen for ______ interactions

Y2H requires little hands-on time and technical skill, making it ______ to use

A limitation of Y2H screens is the high rates of false positives and false ______

For the reporter gene to be activated in Y2H, the interaction must occur in the ______

Protein folding, expression, and post-translational modifications may differ between yeast and humans, affecting their physical ______

Tagged proteins in Y2H may not fold correctly, leading to abnormal binding of their ______

_______ roles of PPIs in cellular functions make them potential therapeutic targets.

Targeted modulation of PPIs with small molecules is a promising approach with more than 50 PPIs successfully targeted with _______ molecules.

Most of the targeted PPIs are _______ of PPIs in drug development.

_______ of PPIs is also a strategy for targeted PPI modulation.

Yeast two-hybrid (Y2H) assay is an established technique to detect protein-protein interactions, and it is often used alongside one or two other methods to examine the _______ of interactions in cells.

The yeast two-hybrid (Y2H) experiment detects physical interactions of proteins through the downstream activation of a _______ gene.

The Y2H assay relies on the expression of a reporter gene activated by the binding of a particular ______

The transcription factor is comprised of a DNA-binding domain (BD) and an activation domain (AD). The protein of interest fused with the BD is known as the ______

In order to activate the reporter gene expression, a transcriptional unit must be present at the gene locus, which is only possible if Bait and Prey ______

Expression of a reporter gene requires the binding of a transcription factor, which normally consists of two functionally and structurally independent domains: DNA-binding (DB) and activation (AD) ______

In the absence of Bait-Prey interaction, the AD domain is unable to localize to the reporter gene to drive gene ______

When Bait and Prey interact, the DB domain binds to the DNA localizing the AD domain upstream of the reporter gene, leading to the expression of reporter ______