term 1 analytical
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Questions and Answers

The image (above) is a depiction of the electromagnetic radiation spectrum. The labels I, III and VI are (in that order):

A I (Decreasing energy), IV (X-rays) and VI (Infrared) B I (Increasing wavefunction), IV (Gamma rays) and VI (Ultraviolet light) C I (Increasing energy), III (Gamma rays) and VI (Infrared) D I (Decreasing energy), III (Radio waves) and VI (Ultraviolet light) E I (Increasing energy), III (X-rays) and VI (Radio waves)

  • I (Increasing energy), III (Gamma rays) and VI (Infrared) (correct)

    • High Performance/Pressure Liquid Chromatography (HPLC) can be used either in normal phase or reverse phase. Which one of the statements below is incorrect?

  • Reverse phase HPLC mobile phase is usually a mixture of water and organic solvents
  • In reverse phase HPLC, the bond strength between analyte and stationary phase is inversely proportional to the analyte hydrophobicity (correct)
  • In reverse phase HPLC, adjusting the pH of the mobile phase will change the hydrophobicity of the analytes
  • In normal phase HPLC, the analyte separation is based on the degree of polar-polar interactions between analyte and stationary phase

    • Which one of the statements (below) regarding discontinuous gel electrophoresis of proteins is incorrect?

  • The proteins are separated on the basis of size
  • The electrical current is applied in cycles of (x) minutes on, (x) minutes off (repeat) – the value of x depends on the molecular weight of the largest protein (correct)
  • The samples are loaded into stacking gel wells
  • The proteins concentrate at the stacking gel/running gel boundary when the electrical current is applied

    • Mammals and birds are frequently used in antibody production. Which of the following animal/animal, animal/bird, bird/animal or bird/bird pairings is/are suitable to act as antibody-producing host/antigen-producing source? (Select all that apply):

    <p>Mouse/goatRat/rabbit D Chicken/hamster (A)</p> Signup and view all the answers

    You are preparing a sample of cells for flow cytometry analysis by staining with the nuclear dye DAPI. Why do you not need a microtome to prepare the sample?

    <p>A microtome is typically used to cut sections from an embedded sample of tissue (C)</p> Signup and view all the answers

    In flow cytometry, laser light striking a cell and being deflected at approximately 90° is called _____________ and is a measure of ______________.

    <p>side scatter cell granularity (A)</p> Signup and view all the answers

    The MIQE guidelines were published in 2009 to standardise the reporting of qPCR experiments in the scientific literature. The acronym "MIQE" stands for:

    <p>Minimum Information for Publication of Quantitative Real-time PCR Experiments (D)</p> Signup and view all the answers

    Which of the following two statements are incorrect about Raman spectroscopy?It uses light from the infrared region of the electromagnetic spectrum

    <p>It uses high energy photons of light to promote electrons within atoms to higher energy levels/It uses light from the ultraviolet region of the electromagnetic spectrum (A)</p> Signup and view all the answers

    The image below shows three types of rotor used in centrifuges. Match the name of the rotor with the image

    <p>A: Fixed angle rotor. B: Swinging-bucket rotor. C: Vertical tube rotor (A)</p> Signup and view all the answers

    A patient has the disorder Congenital Sucrase-Isomaltase Deficiency (CSID). They are unable to catabolise:

    <p>Sucrose (B)</p> Signup and view all the answers

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