APLN - BLOOD BANKING

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156 Questions

The interval between blood donations is:

8 weeks

The oral temperature of a donor shall not:

37.5C

The minimum acceptable hemoglobin for male donors is:

12.5 g/dL

What is the lowest acceptable hematocrit for female blood donors?

38%

Prospective donors who have malaria should be deferred:

For three years after cessation of treatment

The name given to the infectious virus of hepatitis B (HBV) is:

Dane particle

Symptom-free donors immunized with oral polio, measles(rubeola), or mumps vaccines are acceptable after a period of:

2 weeks

For autologous blood donation, blood should not be drawn from the donor patient within ____ hours of the time of the anticipated operation or transfusion.

72 hours

An autologous blood donor must:

Have at least an 11g/dL hemoglobin

Which of the following pretransfusion tests "must" be determined for an autologous transfusion recipient?

ABO and Rh type

Which biochemical change does NOT occur in stored blood?

Plasma Potassium (K) decreases

Which of the following organism have been implicated in bacterial contamination of donor blood?

Bacillus species

The acceptable temperature range for a blood bank refrigerator is:

1-6C

Fresh frozen plasma that is store at 18C or lower has a shelf life of _____.

1 year

When a blood product is stored at 1-6C and the hermetic seal has been broken, the expiration date becomes:

24 hours

The optimum temperature for thawing fresh frozen plasma is:

30-37C

According to FDA regulations, if blood is to be transported, the blood temperature must be kept between:

1-10C

Which blood group system was discovered first?

ABO

An amorph is gene:

With no directly observable product

Production of more antigen in the homozygous state than in the heterozygous state is referred to as:

Dosage effect

Unexpected antibodies that react at 37C are:

Considered most clinically significant

Naturally occurring antibodies are found regularly in which system?

ABO

Select the INCORRECT statement below. Naturally occurring antibodies;

Are usually IgG

Immune antibodies may be produced by:

AOTA

Anti-A1 is a seed extract from:

Dolichos biflorus

The frequency of Group A individuals in the United States is:

41%

Group B individuals have:

Anti-A in their serum

Red blood cells of which blood group will not be agglutinated by anti-A,B typing serum?

O

Red blood cells of which of blood group react most strongly with anti-H?

O

The reagent made from the seeds of Dolichos biflorus is:

Anti-A1

The antigen most commonly tested for in the Rh system is:

D

Which of the following is true of D^u?

Du is a variant of the Rho(D) antigen

Which is not true of Du antigen?

Du cells must be incubated at 4C for one hour

Anti-C will not react with:

rh cells

Anti-E will react with:

Rh2 Rh2 cells

Anti-c can be formed by persons with the genotype:

R1 R1

Anti-hr will NOT react with:

Rh1 Rh1 cells

Anti-hr will react with:

Rh1 Rh1 cells

The f antigen refers to:

ce

The symbol for the Bombay blood group is:

Oh

All but one of the following antibodies will be detected in the serum of Bombay genotype:

Anti-Oh

The system composed of antigens found primarily in saliva and plasma is:

Lewis

In the Duffy blood group system, the most common antibody detected is:

Anti-Fya

Which of the following is usually IgG, immune, and antiglobulin (Coombs') reactive?

Anti-Jka

The antigen I is:

Poorly developed on cord red blood cells

Anti-I is most commonly detected in which crossmatch phase?

Room temperature

Select the incorrect statement:

Anti-I reacts best with cord blood cells

Which of the following is NOT TRUE of anti-Le^a

Is commonly implicated in hemolytic disease of the newborn (HDN)

What percentage of the population lacks the Kell (K) antigen?

90.00%

Select the INCORRECT statement:

Anti-K reacts best in saline at room temperature

Anti - K is also known as

Anti-Cellano

Approximately what percentage of blacks have the Fy (a-b) phenotype?

22%

Recent findings suggest there is an association between the Fy (a- b-) phenotype and resistance to:

Malaria

The antigen Tj^a is part of which system?

P

Approximately what percentage of males are Xga positve?

65.6%

Approximately what percentage of females are Xg^a positive?

11.3%

What color-coding is used for donor blood labels, group A is:

Yellow

What is the standard acceptable color for anti-A grouping serum?

Blue-green

Polyspecific antiglobulin serum is sometimes colored:

Green

What is the color of the dye added to commercially prepared anti-B blood grouping serum?

Yellow

Anti-A,B serum:

Is used to confirm group O individuals

Which of the following is NOT a suitable medium for suspensions of red cells for blood banking?

Distilled water

Which of the following enzymes is NOT used in blood bank procedures?

Amylase

Enzymes prevent detection of antibodies in the:

Duffy system

The technique used to remove antibody bound to sensitized red cells is called:

Elution

A cold autoagglutinin will usually have specificity for the:

I antigen

Paroxysmal cold hemoglobinuria is often associated with antibodies in which system?

P

Forward grouping is using:

Anti-A and anti-B antisera to detect cell antigens A and B

Reverse grouping difficulties may be encountered in the following instances:

AOTA

A patient that forward groups as group B probably is a group A2 B with

Anti-A1

The most dangerous and unexpected antibodies in the blood bank are those that react at:

37C

Antiglobulin reagent:

May be produced in laboratory animals

Which of the following drugs will NOT cause a positive direct antiglobulin (Coombs') test?

Aspirin

What is the optimum incubation temperature for the indirect antiglobulin (IAT) test?

37C

The direct antiglobulin test (DAT) using polyspecific antihuman globulin serum is NOT of value in the:

Differentiation of cell surface coating (complement of IgG)

Albumin-reactive IgG antibodies are about _______ in length:

250 angstroms

By common usage, a polyspecific anti-human serum at least contains antibodies to:

IgG and C3d

The direct antiglobulin test is most useful for:

Detection of hemolytic disease of the newborn

In a direct antiglobulin test, you are testing patient:

Cells

An indirect antiglobulin test is incubated for:

15-60 minutes at 37C

The red blood cells used for screening patients' serum for unexpected antibodies should be of what group?

Group O

A false positive antiglobulin test can be caused by:

Bacterial contamination of reagents

A false negative indirect antiglobulin test can be caused by:

Inadequate washing

Laboratories must check each negative antiglobulin test using red blood cells sensitized with IgG. After the addition of these cells, which of the following is NOT necessarily true?

Patient serum was added

Fresh serum (less than 48 hours old must be used for compatibility testing to preserve:

Complement

Mixed field agglutination is observed under the microscope as clumps or agglutinates of cells among many free cells. Which of the following is the MOST COMMON REASON for mixed field agglutination?

Transfused cells mingling with patient cells

The term "type and screen" refers to:

Testing the patient's blood for ABO and Rh type and unexpected antibodies

All but one of the following antibodies will usually be detected in the room temperature phase of crossmatch:

Anti-Jk a

A positive antigen-antibody reaction is indicated by:

Agglutination or hemolysis

Which of the following antibodies usually gives negative reactions in enzyme test procedures?

Anti-Fya

One of the eight units crossmatched is incompatible in the antiglobulin phase of testing. The most likely antibody is:

Anti-K

If a recepient is incompatible with only one donor, the most probable cause is:

A recipient antibody to a low incidence antigen

Hemolysis requires complement activation. Which of the following are required for complement activation?

Calcium and magnesium

The initial response to a foreign antigen is known as the "primary response". His primary response usually takes how long to occur?

2-6 months

Secondary response to the same foreign antigen is known as:

Anamnestic response

Platelet concentrates are most likely to be of benefit in:

Thrombocytopenia

Cyroprecipitated antihemophilic factor (AHF) is not recommended for the treatment of:

Hemophilia B

Red blood cells are the product of choice for:

Increasing oxygen carrying capacity

Deglycerolized frozen red blood cells must be transferred within how many hours?

24

Recurrent nonhemolytic febrile transfusion reactions are usually caused by all of the following except:

Red cell antibodies

A patient is suspected of having a transfusion reaction. When should the transfusion be stopped?

Immediately

A transfusion reaction is considered febrile when the patient experiences a:

2 degree F rise in temperature within 1 hour of transfusion

The blood phenotype O can result from which of the following genotypes?

OO

The cells and serum of a neonate were tested at room temperature: Patient cells with: Anti-A - negative Anti-B - Positive

       Patient serum with:
                 A1 cells - negative
                 Anti - B - positive

             The infant is probably:

Group B

Patient cells with: Anti-A - positive Anti-B - positive Anti-AB - positive Anti-A1 - negative

Patient serum with: A1 cells - positive B cells - negative

Give the most likely explanation for the discrepancy in the forward and reverse groupings:

The patient is group A2B with anti-A1

What is the optimum concentration of a cell suspension to be used in blood banking testing?

2-5%

The Weiner genotype R2 R2 is equivalent to:

cDE/cDE

What is the most common genotype that could result from the phenotype D,C,E,c,a?

R2R2

Given the reactions, D+C+E+, c+, e+, what is the most probable genotype?

R1r

Given the reactions, D, C, E , ce+, ce+, what is the most

R1R2

If cells are positive when tested with anti-D, anti-C, and anti-e, and negative when tested with Anti-E and anti-c, what is the most likely genotype?

R1R1

If red cells give positive reactions when tested with anti-Rho, anti-rh', anti-hr', and a negative reaction with anti-rh", which of the following genotypes is IMPOSSIBLE?

R1R2

113 Deleted answer a

Deleted

What is the most common of the Rh negative genotypes?

cde/cde

When red cells are positive when tested with anti-c and anti-e and negative when tested with anti-D anti-C and anti-E, what is the genotype?

r r

D^u antigen are fairly common to what race?

Blacks

The D antigen is responsible for the most frequent production of unexpected antibodies. Which other blood group antigen is responsible for a large percentage of unexpected antibody production?

K antigen

Hemolytic disease of the newborn can occur when:

The mother lacks an antigen the infant possesses

Which antibody is most likely to be involved in hemolytic disease of the newborn?

Anti-c

When severe hemolytic disease of the newborn is due to an unidentified antibody:

Mother's red cells may be used for transfusion

The following blood groups, which one does not usually cause hemolytic disease of the newborn?

P

In a case of hemolytic disease of the newborn, when the mother is group O and the baby is group A, red cells for exchange transfusion should be what group?

O

In cases of ABO hemolytic disease of the newborn, the mother is usually which of the following?

Group O

If a mother passes an antibody to her fetus through the placenta, what type of immunity does the fetus have?

Naturally acquired passive

If the mother is group A1B and the father is group A2B, which would be IMPOSSIBLE in the offspring?

A2

In the phenotype mating A2B x 0, which of the following phenotype is possible in the offspring?

B

In the phenotype mating O x O, which of the following phenotypes is possible in the offspring?

O

An A1B mother and an A2O father could NOT produce which of the following genotypes?

A2O

If the mother is group O and the infant is group B, the infant's blood group genotype is:

BO

Given a mother of group O and an infant of group B, which of the following blood groups would eliminate paternity?

O

If a group AB is mated with a group O, the following genotypes will result:

AO and BO

In the mating of R1r x R2r genotypes, which of the following is probably NOT that of the offspring?

CDe/cdE

If an R2R2 patient is transfused with R1R1 blood, it would be possible for him to form which of the following antibodies?

Anti-Ce

If a patient requiring a blood transfusion has an anti-e(hr") approximately what percent of donor blood tested would be compatible?

2%

Approximately what percentage of donor blood is compatible with a patient who has anti-Jka?

25%

You must find two units of compatible blood for a patient who has anti-Jka. How many units will you crossmatch?

10

When a patient is to undergo heart bypass surgery, which antibody is considered clinically significant?

Anti-P1

Which blood grouping antibodies can be the cause of a severe delayed hemolytic transfusion reaction?

Kidd

In titration of an antibody, the first 2-3 tubes are negative and later tubes are positive. This is known as:

Prozone effect

Intravascular hemolysis occur when:

Complement is activated

What is the optimum pH at which antibodies bind to cells?

6.5 - 7.5

A blood bank has 10 units of O Rho (D) positive blood from random donors. It should be easiest to find two units of compatible blood for the patient with:

Anti-Lea

If the patient with anti-E needs a blood transfusion, approximately what percentage of donor blood tested will be compatible?

70%

A patient needing a blood transfusion is found to have anti-P1. According to the statistics, select the appropriate number of compatible units

1 of 4 units

A 56C water bath is commonly used for: 1. Crossmatching 2. Preparing eluates 3. Inactivating sera 4. Thawing frozen cells

2 and 3

Testing media for antigen-antibody reactions include: I. Isotonic saline II. LISS III. Albumin IV. Enzymes

I, II, III, IV

Anti-A1 agglutinin is:
I. Found in group A1 patients II. Occasionally found in A2 patients III. Found in all A2B patients IV. Found in group B and O patients

II and IV

Standardize techniques for blood banking procedures by:

AOTA

Anti-AB serum: I. Is obtained from A1B individuals II. Is obtained from A2B individuals III. Is obtained from group O individuals IV. Confirms group O individuals

III and IV

Rh antibodies: I. React more strongly at 37C than at 4C II. Frequently noted to be cold agglutinins III. Acquired from transfusion of pregnancy IV. Confirms group O individuals

I, II, and III

Which of the following are naturally occurring? I. Anti-P1 II. Anti-Lea III. Anti-N IV. Anti-S

I, II and III

The group B phenotype can result from the following genotype/s? I. A1B II. BB III. AO IV. BO

II and IV

The phenotype A can result from which of the following genotypes?

A1A2 and A1O

In the very acute phase of hepatitis, which of the following would be indicative of the onset of a viremic state?

Positive HBsAg

What is the expiration date (in days) of red blood cells preserved with citrate-phosphate-dextrose-adenine (CPDA-1)?

35

Low ionic strength solution (LISS): I. Causes an increase in the electropositive cations surrounding the red cells II. causes a decrease in the electropositive cations surrounding the
red cells III. usually consists of phosphate saline and glycine IV. enhances the rate and sensitivity of antigen-antibody reactions

II, III and IV

Test your knowledge of blood donation guidelines with this quiz covering topics such as interval between donations, temperature restrictions, acceptable hemoglobin levels, deferral criteria for malaria, and infectious virus of hepatitis B.

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