Questions and Answers
What does the ocular lens do?
What connects the ocular lens to the objective lenses?
Body tube
Which part of the microscope should be held when carrying it?
Arm
The coarse adjustment knob should be used at high power.
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What is the function of the fine adjustment?
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What holds the objective lens and allows for magnification change?
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What is the magnification of the low power objective lens?
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What is the total magnification if the ocular lens magnification is 10x and the objective lens magnification is 40x?
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Where is the stage located on a microscope?
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What holds the slides in place on the stage?
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What adjusts the amount of light reaching the specimen on a microscope?
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What part of the microscope supports its weight?
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What functions as a light source in a microscope?
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What is the role of the condenser lens?
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How should you carry a microscope?
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Who is known as the father of microscopy?
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Study Notes
Microscope Parts and Functions
- Ocular Lens: Eyepiece that magnifies the image 10x as projected by the objective lens.
- Body Tube: Connects the ocular lens to objective lenses, ensuring proper alignment of light from specimen to viewer's eye.
- Arm: Supports the body tube and connects to the base; important for safe handling of the microscope.
- Coarse Adjustment Knob: Used first for major focus adjustments under low power; improper for high power use.
- Fine Adjustment: Provides precise focusing under high power; essential for clear viewing of details.
- Rotating/Revolving Nosepiece: Holds objective lenses; can rotate to change magnification easily.
- Objective Lenses: Includes low, medium, and high power lenses for varying magnification levels—adjustable system.
- Low Power Lens: Lowest magnification at 4x, ideal for initial viewing of specimens.
- Medium Power Lens: Offers medium magnification of 10x, useful for detailed examination.
- High Power Lens: Highest magnification at 40x, designed for viewing fine details in specimens.
- Stage: Platform for slides with a hole to allow light to illuminate the specimen from below.
- Stage Clips: Secure slides in place on the stage to prevent movement during observation.
- Diaphragm: Rotating dial adjusts light intensity reaching the specimen; crucial for optimal visibility.
- Base: Provides stability and support for the microscope; houses the light source and electronics.
- Light Source/Illuminator: An electric light or mirror that directs light upwards towards the specimen.
- Condenser Lens: Focuses light from the illuminator through the stage’s hole onto the specimen for better clarity.
- Proper Carrying Technique: Always support the microscope by the arm while holding the base with the other hand.
- Anton van Leeuwenhoek: Recognized as the father of microscopy; discovered bacteria using simple microscopes.
- Total Magnification Formula: Calculated by multiplying the magnification of the ocular lens by the magnification of the objective lens.
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