Lecture 11 – Bacterial transcription
29 Questions
1 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the primary role of transcription in gene expression?

  • To convert DNA sequences into RNA. (correct)
  • To modify proteins after translation.
  • To replicate DNA.
  • To translate RNA into proteins.
  • Which RNA molecule serves as the template for protein synthesis?

  • tRNA
  • mRNA (correct)
  • snRNA
  • rRNA
  • In bacterial transcription, which strand of DNA serves as the template strand?

  • `Coding strand
  • Sense strand
  • Antisense strand (correct)
  • Non-template strand
  • During transcription, which nucleotide replaces thymine in RNA?

    Signup and view all the answers

    What is the consequence of cytosine deamination in DNA?

    <p>It becomes uracil, potentially leading to mutations.</p> Signup and view all the answers

    What is the function of the σ subunit in bacterial RNA polymerase?

    <p>To facilitate the binding of RNA polymerase to promoter regions.</p> Signup and view all the answers

    What defines a polycistronic operon in bacterial transcription?

    <p>Multiple genes transcribed as a single mRNA molecule.</p> Signup and view all the answers

    Which element of the bacterial promoter is typically recognized by RNA polymerase?

    <p>Promoter regions</p> Signup and view all the answers

    In DNA footprinting, what does the “footprint” represent?

    <p>The region of DNA that is protected from nuclease digestion.</p> Signup and view all the answers

    Which sequences are characteristic of a strong bacterial promoter?

    <p>-10 and -35 sequences that closely match consensus sequences.</p> Signup and view all the answers

    What is the initial step in the transcription process?

    <p>Elongation of the RNA strand.</p> Signup and view all the answers

    During the elongation phase of transcription, what happens to the RNA:DNA hybrid?

    <p>It remains stable for a short length of the transcript.</p> Signup and view all the answers

    Which mechanism is involved in rho-independent transcription termination?

    <p>Hairpin loop formation followed by poly-U stretch.</p> Signup and view all the answers

    What is the significance of the ω subunit in bacterial RNA polymerase?

    <p>It enhances the stability and assembly of the enzyme.</p> Signup and view all the answers

    What distinguishes RNA polymerase from DNA polymerase?

    <p>RNA polymerase synthesizes RNA without a primer.</p> Signup and view all the answers

    What is a common feature of bacterial transcription units?

    <p>They may contain multiple genes organized in operons.</p> Signup and view all the answers

    Which of the following is true regarding the termination of transcription in bacteria?

    <p>It always involves ribosome activity.</p> Signup and view all the answers

    What is the typical rate of RNA elongation by bacterial RNA polymerase?

    <p>Approximately 50 nucleotides/sec</p> Signup and view all the answers

    Which of the following statements about σ factors is correct?

    <p>Different σ factors recognize different promoter sequences.</p> Signup and view all the answers

    What triggers the transition from the closed promoter complex to the open promoter complex?

    <p>Unwinding of the -10 region of DNA</p> Signup and view all the answers

    How does the presence of a hairpin structure in RNA contribute to transcription termination?

    <p>It destabilizes the RNA polymerase-DNA complex.</p> Signup and view all the answers

    What is the role of topoisomerase during transcription?

    <p>To relieve the supercoiling of DNA.</p> Signup and view all the answers

    Which of the following is true regarding the -35 and -10 consensus sequences?

    <p>They are conserved elements that help RNA polymerase bind effectively.</p> Signup and view all the answers

    What is the primary function of ribosomal RNA (rRNA)?

    <p>To form the structural and functional components of ribosomes.</p> Signup and view all the answers

    Which type of RNA is specifically involved in bringing amino acids to the ribosome during translation?

    <p>tRNA</p> Signup and view all the answers

    What happens to the RNA polymerase once transcription is terminated?

    <p>It dissociates from the DNA and newly synthesized RNA.</p> Signup and view all the answers

    What is the effect of having a strong promoter sequence in bacterial transcription?

    <p>It increases the efficiency of RNA polymerase binding and transcription initiation.</p> Signup and view all the answers

    What is the typical structure of bacterial RNA polymerase?

    <p>A multi-subunit complex.</p> Signup and view all the answers

    What is the significance of the +1 position in a bacterial promoter?

    <p>It indicates the start of transcription.</p> Signup and view all the answers

    More Like This

    lecture 11 on land plants
    121 questions

    lecture 11 on land plants

    IllustriousMahoganyObsidian avatar
    IllustriousMahoganyObsidian
    Lecture 11
    15 questions

    Lecture 11

    WellKnownElation avatar
    WellKnownElation
    Use Quizgecko on...
    Browser
    Browser