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Questions and Answers

What is the basic building block of nucleotides?

A nucleoside

DNA is the first three-dimensional Xerox machine.

True (A)

What is the name of the enzyme responsible for unwinding the DNA template at the replication fork?

Helicase

What are the three types of DNA repair?

<p>Double-strand break repair (A), Single-strand break repair (B), Mismatch repair (C)</p> Signup and view all the answers

Telomerase only operates in prokaryotes.

<p>False (B)</p> Signup and view all the answers

What are the 3 types of single-nucleotide mutations?

<p>Transition, transversion, and frameshift</p> Signup and view all the answers

What are the three main types of RNA polymerase found in eukaryotes? What does each produce?

<p>RNA polymerase I produces rRNA, RNA polymerase II produces mRNA (as well as microRNA and snRNA), and RNA polymerase III produces 5S rRNA, tRNA.</p> Signup and view all the answers

What are the five types of genetic code features?

<p>Unambiguous, degenerate, nonoverlapping, commaless, and universal.</p> Signup and view all the answers

What are the three primary mechanisms of epigenetic change?

<p>DNA methylation (A), Histone modification (B), Non-coding RNA (C)</p> Signup and view all the answers

Anti-U1 snRNP antibodies are associated with various autoimmune diseases.

<p>True (A)</p> Signup and view all the answers

What does the term ‘alternative splicing’ refer to? What are some clinical examples?

<p>Alternative splicing is the process by which different exons are frequently combined to produce a larger number of unique proteins. This can be seen in the creation of a transmembrane immunoglobulin vs a secreted immunoglobulin, tropomyosin variants in muscle, and dopamine receptors in the brain.</p> Signup and view all the answers

The CCA at the 3' end of tRNA is necessary for amino acid binding to the tRNA.

<p>True (A)</p> Signup and view all the answers

What are the three types of tRNA binding sites on a ribosome?

<p>A site, P site, and E site.</p> Signup and view all the answers

Flashcards

Chromatin Structure

DNA is packaged into a condensed form that fits within the nucleus.

Histone

Proteins with a positive charge that DNA wraps around.

Nucleosome

DNA wrapped around a core of histone proteins.

Euchromatin

Less condensed form of chromatin, active in transcription.

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Heterochromatin

Highly condensed chromatin, inactive in transcription.

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DNA Methylation

Changes DNA expression without altering the sequence.

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Histone Acetylation

Relaxing DNA coiling, promoting transcription.

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Histone Deacetylation

Tightening DNA coiling, suppressing transcription.

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Nucleotide

Base + sugar + phosphate; building block of nucleic acids.

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Purines

Adenine and Guanine (2 rings).

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Pyrimidines

Cytosine, Uracil, and Thymine (1 ring).

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DNA Replication

Creating new DNA from existing DNA.

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Semiconservative Replication

Each new DNA molecule contains one original strand.

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Replication Fork

Y-shaped region where DNA is unzipped during replication.

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Helicase

Enzyme that unwinds the DNA double helix.

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DNA Polymerase III

Main enzyme for DNA synthesis during replication (prokaryotes).

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Okazaki Fragments

Short DNA segments synthesized discontinuously on the lagging strand.

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DNA Ligase

Joins the Okazaki fragments together.

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Double-strand break repair

Mechanism for fixing breaks in both DNA strands.

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Homologous recombination

Repairing DNA using a similar template.

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Nucleotide Excision Repair

Fixing bulky DNA damage.

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Base Excision Repair

Fixing damaged DNA bases.

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Mismatch Repair

Correcting mismatched bases in DNA.

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Genetic Code Features

Defines how DNA sequences translate into proteins.

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