Basic Microbiology MLT202 PDF

Basic Microbiology MLT202 Dr. Mohamed Mohsen  Evaluation and Assessment Methods Method(s) Timing Weight Final written Examination 16th Week 50 33.3% Final practical Examination 14th week 50 33.2%...

Basic Microbiology MLT202 Dr. Mohamed Mohsen  Evaluation and Assessment Methods Method(s) Timing Weight Final written Examination 16th Week 50 33.3% Final practical Examination 14th week 50 33.2% Other assignments Q1 4th week 15 10% Q2 8th week 15 10% Q3 12th week 20 13.3% What is Microbiology?? Micro =small, bios =life, logos =science (The study of living things which are too small and cannot seen by the naked eyes) MICROBIOLOGY Identification of microorganisms such as bacteria, fungi, viruses and parasites which cause infection, and testing for effective antibiotics. Microbiology is the study of all living organisms that are too small to be visible with the naked eye  Specimen Collection Guidelines Obtain during Collect before Aspirates or tissues acute phase of Sample antibiotics are are preferred to infection (within 2– appropriate site. administered. swabs. 3 days for viruses). Avoid contamination with Obtain sufficient Use sterile, leak- Properly label environmental or quantity. proof container. container. normal flora. Syringes with For prolonged Deliver to lab within needles attached transit, use special 30 min of should never be preservatives or collection. transported. holding media.  Specimen Preservation and Storage Specimen Preservative or transport device Storage temperature (unpreserved) Urine Transport tubes with boric acid–glycerol if specimen 4° C for not more than 24 hr can’t be processed within 2 hr of collection Stool for ova & Depending on procedures to be performed: Can hold formed specimens at 4° C. parasites polyvinyl alcohol (PVA), 10% formalin, sodium Soft or liquid specimens should be acetate–acetic acid–formalin (SAF), merthiolate- examined immediately or preserved iodine-formalin (MIF), others without formaldehyde or mercury (e.g., Ecofix, Parasafe) Sputum None 4° C Stool for culture Transport media, e.g., Cary-Blair transport medium 4° C. Best to process without delay. if specimen can’t be cultured within 2 hr of Refrigeration may kill Shigella collection  Unlabeled or improperly labeled specimen Improper collection site Prolonged transit (over 2 hr without preservation) Improper temperature during transport or storage Criteria for Leaking specimens Rejection of Specimens in nonsterile containers Improper transport medium Specimens in Culture ampule not broken; swab dried out Microbiology Improper swab, e.g., wood for viruses or Chlamydia Specimen received in formalin (other than stool for ova & parasites) Saliva instead of sputum Insufficient quantity Specimen management Specimen Collection Specimen Transport Specimen Handling Task 1 Specimen Storage Specimen Disposal  Wear protection Never pipette by mouth Wash your hands Do not eat or drink in the Disinfect work areas lab before and after use Avoid skin and eye contact Sterilize equipment and with all chemicals materials  THANK YOU Do you have any questions? [email protected] 01114036631 Must university