Investigating Chlorophyll's Role in Photosynthesis PDF

**Investigating whether chlorophyll is necessary for photosynthesis** **[Laboratory procedure]** 1. Obtain a destarched potted plant with variegated leaves. 2. Pick a leaf from the potted plant. 3. Perform an iodine test on the picked leaf. 4. Leave the plant in bright light for four hours....

**Investigating whether chlorophyll is necessary for photosynthesis** **[Laboratory procedure]** 1. Obtain a destarched potted plant with variegated leaves. 2. Pick a leaf from the potted plant. 3. Perform an iodine test on the picked leaf. 4. Leave the plant in bright light for four hours. 5. Pick another leaf from the plant after the four-hour period. 6. Draw a labeled diagram showing the color pattern of the newly picked leaf. 7. Conduct another iodine test on this leaf. 8. Draw a labeled diagram to show the result of the second iodine test. 1\. After the iodine test, what color change is expected in the green parts of the leaf? a\) Orange-brown b\) Blue-black c\) Yellow d\) No change 2\. What color change is expected in the non-green parts of the leaf after the iodine test? a\) Orange-brown b\) Blue-black c\) Yellow d\) No change 3\. The experiment aims to demonstrate that: a\) Chlorophyll is not necessary for photosynthesis b\) Chlorophyll is necessary for photosynthesis c\) Variegated leaves cannot photosynthesize d\) Iodine is necessary for photosynthesis 4\. The presence of starch in the leaf indicates: a\) Respiration has occurred b\) Transpiration has occurred c\) Photosynthesis has occurred d\) Chlorophyll is absent 5\. The experiment\'s conclusion is expected to show that: a\) Only green parts of the leaf produce starch b\) Both green and non-green parts produce starch c\) Neither green nor non-green parts produce starch d\) Starch production is random in the leaf 6\. What is the purpose of using a destarched plant in Step 1? a\) To ensure the plant is healthy b\) To remove any pre-existing starch in the leaves c\) To increase chlorophyll content d\) To make the leaves more variegated 7\. Why is the initial iodine test performed in Step 3? a\) To check for pre-existing starch b\) To increase chlorophyll content c\) To destarch the plant d\) To activate photosynthesis 8\. What is the purpose of leaving the plant in bright light for four hours in Step 4? a\) To increase leaf variegation b\) To allow time for transpiration c\) To provide conditions for photosynthesis d\) To destarch the plant 9\. Why is a second leaf picked after the light exposure in Step 5? a\) To compare with the first leaf b\) To test for starch production during light exposure c\) To increase sample size d\) To check for leaf damage 10\. What is the purpose of drawing a labeled diagram in Step 6? a\) To practice artistic skills b\) To record the color pattern of the leaf c\) To measure leaf size d\) To count chloroplasts 11\. Why is a second iodine test performed in Step 7? a\) To compare with the first test b\) To check for starch production after light exposure c\) To remove chlorophyll d\) To prepare the leaf for microscopy 12\. What is the independent variable in this experiment? a\) Presence of starch b\) Presence of chlorophyll c\) Amount of light d\) Temperature 13\. What is the dependent variable? a\) Presence of starch b\) Presence of chlorophyll c\) Amount of light d\) Temperature 14\. Which of the following is NOT a controlled variable in this experiment? a\) Supply of carbon dioxide b\) Supply of oxygen c\) Presence of chlorophyll d\) Temperature 15\. Why is the non-green part of the leaf considered the control setup? a\) It contains more chlorophyll b\) It lacks chlorophyll c\) It produces more starch d\) It receives more light 16\. Which factor is deliberately varied between the green and non-green parts of the leaf? a\) Light exposure b\) Carbon dioxide supply c\) Presence of chlorophyll d\) Temperature 17\. Why is it important to control the supply of carbon dioxide in this experiment? a\) To ensure it\'s not a limiting factor for photosynthesis b\) To prevent respiration c\) To increase leaf variegation d\) To change leaf color 18\. Why is temperature controlled in this experiment? a\) To prevent leaf wilting b\) To ensure consistent enzyme activity c\) To change leaf color d\) To increase chlorophyll production 19\. Why is the supply of water controlled? a\) To prevent drowning the plant b\) To ensure it\'s not a limiting factor for photosynthesis c\) To change leaf texture d\) To dissolve chlorophyll 20\. What role does controlling the light supply play in this experiment? a\) To ensure consistent photosynthesis conditions b\) To change leaf color c\) To increase leaf size d\) To break down chlorophyll 21\. What type of plant is suggested for this experiment? a\) Cactus b\) Fern c\) Coleus d\) Pine 22\. Why is a variegated leaf used in this experiment? a\) For aesthetic reasons b\) To provide both chlorophyll-containing and chlorophyll-free areas c\) To increase photosynthesis rate d\) To absorb more light 23\. What chemical is used to test for the presence of starch? a\) Benedict\'s solution b\) Iodine solution c\) Sodium hydroxide d\) Ethanol 24\. What safety precaution is mentioned regarding the use of alcohol? a\) Wear goggles b\) Use it away from naked flames c\) Drink plenty of water d\) Wear a lab coat 25\. How long is the plant left in bright light? a\) 2 hours b\) 3 hours c\) 4 hours d\) 5 hours 26\. What color does iodine solution turn in the presence of starch? a\) Red b\) Green c\) Yellow d\) Blue-black 27\. Why is it important to destarch the plant before the experiment? a\) To make the leaves more colorful b\) To remove any pre-existing starch that could affect results c\) To increase chlorophyll content d\) To make the plant grow faster 28\. What does the presence of starch in a leaf section indicate? a\) Respiration occurred b\) Photosynthesis occurred c\) Transpiration occurred d\) Chlorophyll is absent 29\. Why is it necessary to draw labeled diagrams in this experiment? a\) To practice drawing skills b\) To accurately record observations c\) To measure leaf size d\) To count chloroplasts 30\. What is the main difference between the green and non-green parts of the variegated leaf? a\) Water content b\) Presence of chlorophyll c\) Leaf thickness d\) Cell wall composition