Blood Sample Preparation (Plasma and Serum) and Blood Hemolysis PDF

Blood sample preparation (plasma and serum) and Blood hemolysis Lab 3 ‫صفيه سعد ضيف‬ ‫المرحلة االولى‬ ‫فسلجة عملي‬ Blood collection tubes The tubes are covered with a color-coded plastic cap. They often include additives that mix with the blood wh...

Blood sample preparation (plasma and serum) and Blood hemolysis Lab 3 ‫صفيه سعد ضيف‬ ‫المرحلة االولى‬ ‫فسلجة عملي‬ Blood collection tubes The tubes are covered with a color-coded plastic cap. They often include additives that mix with the blood when collected, and the color of the tube's plastic cap indicates which additives that tube contains. Notice that you shouldn't use the wrong tube may therefore make the blood unusable.  Blood plasma Plasma is a light yellow liquid of blood. It is mainly composed of 92% water mixed with blood proteins and inorganic electrolytes. The most abundant plasma solute is the plasma protein, of which there are three groups: albumin, globulins, and fibrinogen. Materials of Plasma Preparation Disposable gloves Tourniquet Syringe Cotton Alcohol (70%) Plasma blood tube such as EDTA-treated (lavender tops) or citrate-treated (light blue tops) Heparinized tubes (green tops) Plaster  Procedure of Plasma Preparation Draw blood from patient. Select vacutainer with an appropriate anticoagulant. Mix well with anticoagulant. Allow to stand for 10 min. Centrifuge the sample to speed separation and affect a greater packing of cells. The supernatant is the plasma which can be now collected for testing purposes or stored (-20C to - 80C) for subsequent analysis or use. Blood serum When a blood sample is left standing without anticoagulant, it forms blood clot. One of the normal components of plasma is a soluble plasma protein called fibrinogen, this protein will be converted to insoluble substance called fibrin this occurrence is referred to as blood coagulation or clotting. Serum is the liquid portion of the blood after it has been allowed to clot. Serum contains the clotting metabolites products (fibrin) that result from the clotting process. For many laboratory tests, plasma and blood serum can be used. Materials of serum Preparation Disposable gloves Tourniquet Syringe Cotton Alcohol (70%) serum blood tube (red or gold) Plaster  Procedure of Serum preparation Draw blood from patient. Select blood tube with NO anticoagulant. Allow to stand for 20-30min for clot formation. Centrifuge the sample to speed separation and affect a greater packing of cells. Clot and cells will separate from clean serum and settle to the bottom of the vessel. The supernatant is the serum which can be now collected by dropper or pipette for testing purposes or stored (-20C to - 80C) for subsequent analysis or use.  Blood hemolysis Hemolysis means liberation of hemoglobin from RBCs. Due to hemolysis, plasma or serum assumes pink to red color. It is important to avoid hemolysis during sampling**, transporting** and storage (too hot or too cold) because hemolysis causes changes in measurement of a number of analysis. Good Luck