Cells - Biology PDF
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This document provides an overview of cell biology, covering cell structure and the functions of different parts of a cell such as the cell surface membrane, cytoplasm, and nucleus. It also details processes like diffusion, osmosis, and cell specialization. The document is likely meant as instructional material rather than a test.
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Cells ellsarethebuildingblocksoflife.Mostcellshavesimilarparts.Eachlivingcellcontains C protoplasm. Protoplasm Thisismadeupofthreeparts:thecellsurfacemembrane,thecytoplasm,andthenucleus. Belowaretheprotoplasmparts...
Cells ellsarethebuildingblocksoflife.Mostcellshavesimilarparts.Eachlivingcellcontains C protoplasm. Protoplasm Thisismadeupofthreeparts:thecellsurfacemembrane,thecytoplasm,andthenucleus. Belowaretheprotoplasmpartsofcells.Ifit’slimitedtoplantsoranimalsonly,itwillbestated. ❡Cellsurfacemembrane ❡Cytoplasm(Willbefurtherdefined) ❡Nucleus(Willalsobefurtherdefined,calmdown) ❡CellWall(Plantonly) heCellsurfacemembraneisaselectivelypermeablemembranewhichcontrolssubstances T movinginandoutofthecellandactsasreceptorsitesforrecognisingexternalstimuliand chemicals.Itallowssmallersubstancessuchaswatertopassthrough.Itismadeofproteins andphospholipids.Itformsaboundarybetweenthecontentsofthecellandtheexternal environment. Thecytoplasmisanaqueousgroundsubstancecontainingorganellessuchasthenucleus. henucleuscontainshereditarymaterialandDNAintheformofchromatinthreadswhich T condenseintohighly-coiledchromosomesvisibleunderthelightmicroscopeduringcelldivision. Thenuclearmembranesurroundsthenucleoplasm.Thenuclearmembraneconsistsoftwo membranes,oneiscontinuouswiththeendoplasmicreticulum.Thenuclearmembraneis perforatedwithporeswhichcontrolthesubstancesmovinginandoutofit.Thenucleusalso controlsanddirectsactivitiesofthecell,suchascellgrowth. hecellwallisaninflexible,inelastic,andtotallypermeable,andismadeupofcellulose.It T mayundergoextensivelignification.Thecellwasprotectsthecellfromoverextensionand providessupportforthecell. Cytoplasmandorganelles hecytoplasmcontainstheorganellesofthecell.Eachorganelleisspecialisedforaparticular T function. Belowaretheorganellesofacell. ❡Roughendoplasmicreticulum ❡Smoothendoplasmicreticulum ❡Golgiapparatus(andvesicles) ❡Ribosomes ❡Mitochondria ❡Chloroplasts(Plantonly) ❡Vacuole(s) ndoplasmicreticulum(ingeneral)-Networkoffoldedmembranesformingsheets,tubes,or E flattenedsacscalledcisternaeincytoplasm,originatingfromtheoutermembraneofthe nuclearmembrane. heroughendoplasmicreticulumiscoveredwithribosomesontheoutersurfaceofthe T membrane.Ittransportsproteinsmanufacturedbytheribosomeswhicharethenexportedoutof thecell. hesmoothendoplasmicreticulumisnotcoveredinribosomesandismoretubular.It T synthesiseshormonesandsteroids,detoxifiesharmfulsubstancesintoharmlessmaterials,and givesrisetotheGolgibody. hegolgiapparatus/bodyisacompactstackofmembrane-boundsacsandgolgivesicles. T Sacsarefluidfilledandpinchattheendstoformgolgivesicles.Ithasaconvexfacewhere vesiclesfromERfusewiththegolgibody,andaconcavefacewherevesiclespinchoff.It chemicallymodifiessubstancesmanufacturedbytheER,andstoresandpackagesthemfor secretionoutofthecell. heribosomesaremadeofribosomalRNAandproteinsandcanbefoundinthecytoplasmor T attachedtoendoplasmicreticulum.RERribosomessynthesisesecretoryproteins,whilefree ribosomessynthesizeintra-cellularproteins. hemitochondriaisadoublemembraneorganellethatisrod-shaped,releasesenergyinthe T formofATP(AdenosineTriphosphate).(Highlyactivecellsmaypossessupto1000.) hechloroplastshasadoublemembrane.Chloroplastscontainaseriesofmembranes T (thylakoids)runningthroughamatrixwherechlorophyllcanbelocated.Thylakoidscanbe stackedtoformagranum.Containsstarchgrainsconvertedfromglucosefromphotosynthesis. Chloroplastscontainchlorophyllpigmenttoabsorbsunlighttomanufactureglucoseduring photosynthesis. hevacuoleisafluid-filledorganellesurroundedbyaselectivelypermeablevacuolar T membrane.Itprovidessupportforaplantbybeingfilledwithwater,andtemporarilystores wasteproductsandfoodreserves. Cellspecialisation herearemanydifferenttypesofcellsinthehumanbody.Differentiationistheprocessof T newlyproducedcellsdevelopingspecialstructuresorlosingcertainstructurestocarryout specificfunctions. ellspecialisationallowscellstobemoreefficientatitstasks.However,ifanunusualneed C arises,itwillbelessabletoadapttothat. tissueisagroupofcellswithsimilarstructureswhichworktogethertoperformaspecific A function.Simpletissuearecellsofthesamekindwhichgrouptogether.Musculartissueis simpletissue.Complextissuearetissueswhichcontainmorethanonetypeofcell.Bloodisan exampleofcomplextissue. norgancontainsmorethanonetypeoftissuewhichallworktogetherforaspecificfunction. A Thestomachcontainsglandtissue,musculartissue,andnervoustissue. (organ)systemconsistsofseveralorgansworkingtogetherforacommonpurpose.Asystem A inthehumanbodyissomethingsuchasthedigestivesystem. Anorganismisvarioussystemsworkingtogethertomakeuptheentirebodyoftheorganism. edbloodcellsdonotcontainanucleustocontainmorehaemoglobin,nottopackmore R oxygen. Twotypesofcellularorganisationsarerecognised: ProkaryotesandEukaryotes Eukaryotesarecellsthatpossessanucleusandmembrane-boundorganelles. rokaryotesarecellsthatdonotpossessamembraneboundnucleus.Theyaresmallerthan P eukaryotes. Asareminder,plantcellsaren’tnecessarilyrectangular. MovementofSubstances AnOverview hemovementofsubstancesinandoutofcellsacrosstheirplasmamembraneshastooccur T continuouslyforthefunctioningoflivingtissues. Thepurposeofthatisto: ❡Allowentryofsubstancesusefultothecellsuchasoxygen ❡Removeexcretoryproductsfromthecellsuchascarbondioxide ❡Secretionofsubstancesoutofthecellsuchashormones CellSurfaceMembrane|FluidMosaicModel hereisabilayerofphospholipids,withhydrophobictailsfacinginwards.Phosphilipidsmove T withinthebilayer,andinmobileproteinsareembeddedorattachedtowithinthebilayer. hospholipidshaveahydrophilicheadandahydrophobictail.Thephospholipidsalsocontain P cholesterol. Therearetwotypesofmembraneproteins:Integral/IntrinsicandPeripheral/Extrinsic. Intrinsicmembraneproteinsaretightlyboundtothephospholipidbilayer.Hydrophobicamino acidsareincontactwiththehydrophobiclipidsonthebilayer.Hydrophilicproteinsareexposed totheaqueousmediumoneithersideofthemembrane. xtrinsicmembraneproteinsarelooselyattachedtothemembranesurface,totheexposed E portionsofintegralproteins. Thereare5functionsofmembraneproteins. ❡Transport ❡Enzymaticreactions ❡Signaltransduction ❡Celltocellrecognition ❡Intercellularjoiningandadhesion ransportproteinsareinvolvedinthemovementofspecificmoleculesacrossthemembraneby T actingaseitherchannelorcarrierproteins. nzymaticreactionproteinshavetheiractivesitesexposedtosubstancesoneithersideofthe E membrane.Insomecases,theenzymesareorganisedaspartofametabolicpathway. ignaltransductionproteinsactasreceptorstoreceivechemicalmessengerssuchas S hormones.Uponbindingandreceivingthesignal,theproteinwillundergoaconformational changetorelaythemessagetotheinsideofthecell. Forthethreeroutesthatthingscanpassthroughtheplasmamembraneofacell: hroughthephospholipidbilayerisforsubstancesthatarefat-solubleandnotrepelledby T thehydrophobiclayer. Throughthechannelproteinsareforwater-solublemoleculesonly. hroughthecarrierproteinsareforwater-solubleandwater-insolublemolecules.Itsupports T chargedparticles(Na+,Ca2+)andrelativelylargepolarmolecules(aminoacids,glycerol). hannelproteinsarecategorisedunderfacilitateddiffusion.Itdoesnottakeupenergy.Itonly C transportssubstancesdowntheconcentrationgradient. arrierproteinsarecategorisedunderactivetransport.Ittakesupenergy,butonlywhenthe C transportationofsubstancesgoesagainsttheconcentrationgradient. Carrierismuchslowerthanchannel. Diffusion iffusionisthenetmovementofsubstancesfromoneplacetoanotherplacedowna D concentrationgradient.Itiscausedbyparticles(atoms,molecules,ions)bumpingintoeach otherandspreadingoutwards. InasituationwherePointAisaplacewhereaperfumebottlewasspilledandPointBisaplace wheresomeoneisexisting.GasparticlesaremoreconcentratedatPointAthanatPointB. Thereisadifferenceinconcentration.Thisistheconcentrationgradient.Thelargerthe difference,thesteepertheconcentrationgradient,andthefasterparticleswoulddiffusefrom PointAtoPointB. ellsatthelungsexchangeoxygenandcarbondioxidethroughtheprocessofdiffusion,andso C doroothaircells. Osmosis smosisisthenetmovementofwatermoleculesfromasolutionofhigherwaterpotentialtoa O solutionoflowerwaterpotential,throughapartiallypermeablemembrane.“Waterpotential”isa measureofthetendencyofwatertomovefromoneplacetoanother.Adilutesolutioncontains morewatermoleculesperunitvolumethanaconcentratedsolutionhenceadilutesolutionhas ahigherwaterpotentialthanaconcentratedsolution.Awaterpotentialgradientisestablished whenapartiallypermeablemembraneseparatestwosolutionsofdifferentwaterpotentials. upposingthatthereisacontainerwithtwosegmentsseparatedbyapartiallypermeable S membraneandthattheleftsideisfilledwitha10%sucrosesolutionwhiletherightsideisfilled witha5%sucrosesolution,waterwillmovetotheleftsideofthecontainerthroughosmosisdue totherightsidehavingahigherwaterpotentialthantheleftside.Itcanbesaidthattherewasa netmovementofwaterfromaregionofhigherwaterpotentialtoaregionoflowerwater potentialdownthewaterpotentialgradient. SurfaceAreatoVolumeRatio+L ssomethingincreasesinvolume,thesurfaceareawouldincreaseataslowerratethanthe A volumesincesurfaceareaisin,forexample,metresquared,whilevolumeissomethingsuchas metrecubed.Cellswillnotgrowpastacertainsizebecauseofthis. ActiveTransport hisistheprocessinwhichenergyisusedtomovetheparticlesofasubstanceacrossa T membraneagainstitsconcentrationgradientfromaregionoflowerconcentrationtoaregionof higherconcentration. ctivetransportisperformedbyspecificcarrierproteinsinthemembrane.Activetransport A enablesacelltomaintaininternalconcentrationsofsmallmoleculesthatdifferfrom concentrationsinitsenvironment.Forexample,comparedtoitssurroundings,ananimalcell hasamuchhigherconcentrationofK+ionsandlowerconcentrationofNa+ions.Thecarrier proteinknownassodium-potassiumpumpthereforeismaintainingthesesteepgradientsby pumpingNa+outofthecellsandK+intothecells. CellsandWaterpotential Ifananimalcellhasahigherwaterpotentialthanthesurroundingsolution,waterwillleavethe cellbyosmosisthroughtheselectivelypermeablemembrane.Thecellwillshrink,andspikes appearonthecellmembrane.Thisiscalledcrenation.(Cremationiswhenahumanbody’scells undergoestoomuchcrenationanddies,andthenbodygettingburied) Ifaplantcellhasahigherwaterpotentialthanthesurroundingsolution,thesamewillhappen. Thelargecentralvacuoledecreasesinsize,causingthecytoplasmtoshrinkaswellandthecell membranepullingawayfromthecellwall.Thisiscalledplasmolysis. Ifananimalcellhasalowerwaterpotentialthanthesurroundingsolution,waterwillenterthe cellbyosmosisthroughtheselectivelypermeablemembrane.Thecellwillexpandandburst.It happensbecausethecellmembranerupturesandthereisnocellwall.Thisiscalledlysis.(The cellislysed.) Ifaplantcellhasalowerwaterpotentialthanthesurroundingsolution,waterwillenterthecell byosmosisinthesameway.Thelargecentralvacuolewillincreaseinsize,causingthe cytoplasmtobepushedagainstthecellwall,whichmakesthecellturgid.Thecellwallprevents itfrombursting.Thereisn’treallyanameforthissoI’mgonnacallthisturgorpressure. BulkTransport argemoleculessuchasproteinsandpolysaccharidescrossthecellmembraneinbulkby L mechanismsthatinvolvepackagingintovesicles.Theseprocessesrequireenergy.Thereare twotypesofbulktransport: ❡Endocytosis ❡Exocytosis Exocytosis Duringexocytosis,transportvesicles(calledsecretoryvesiclesorGolgi vesicles)thatbuddedfromtheGolgiApparatusmovetowardsthecell surfacemembrane. Whenthevesiclemembraneandplasmamembranecomeintocontact, thetwomembranesfuse.Thecontentsofthevesiclesarereleasedand thevesiclemembranebecomespartoftheplasmamembrane. Extocytosisinvolvesvesiclesalot. Endocytosis ndocytosis Duringendocytosis,particlesaretakenintothecellsthroughthe E formationofnewvesicles.Particlesthatareabouttobetakeninorengulfedcomeinto contactwiththecellsurface.Thecellmembraneinvaginates(foldsinwards)toforma flask-likedepressionaroundtheparticlesorthecellmembraneextendsoutwards formingextensions(pseudopodia)aroundtheparticles.Subsequently,thedepression deepensandtheneckoftheflaskclosesandsealsofftheinvagination,forminga separatevesicle Therearethreetypesofendocytosis.Theyare: ❡Phagocytosis ❡Pinocytosis ❡Receptor-mediatedendocytosis hagocytosisreferstotheprocesswhichtakesupmaterialsinsolidform(suchasbacteriaor P cells).Particleswithintheformedvesicleusuallygetdigestedbytheenzymesinthecell. hagocytosisisaselectiveprocess,meaningthatcellswillonlytakeupspecificparticles. P Certainwhitebloodcellsengulfbacteria. inocytosisreferstotheprocesswhichtakesupmaterialinliquidform,andthisis P non-selective. eceptor-meditatedendocytosisreferstotheprocessinwhichmaterialistobetakenupby R bindingtothereceptorproteinsonthecellmembrane.Thisisaselectiveprocess. BiologicalMolecules-Nutrients Nutrientsarecategorisedintomultiplethings,itincludesthingssuchasfoodandwater. Foodisneededto: ❡Provideenergyforvitalactivities ❡ Supplyrawmaterialstomakenewprotoplasmforcellgrowth,reproduction,andrepairfor worn-outtissues ❡Helporganismsstayhealthy ❡Sustainone’sdrugaddiction herearethreetypesofnutrients.Nutrientsarechemicalsubstancesinthefoodthatweeat.It T includescarbohydrates,fats,andproteins. Waterisvitalforallbodytissuesinanorganism.Waterisneededto: ❡Beasolventforchemicalreactions ❡Beakeycomponentoftissues ❡Controlbodytemperature ❡Transportingdissolvedsubstances atertransportsdissolvedsubstancesastheroleofasolvent.Thedissolvedsubstances W includedigestedproductsandwasteproducts. Inplants,water ivingorganismsaremadeupofchemicalsbasedmostlyoncarbon.Waterlackscarbonandis L inorganic.Carbonentersthebiospherethroughphotosynthesiswherecarbondioxideinthe atmosphereisconvertedtocarbohydrates. arbohydrates,Lipids,Proteins,andNucleicacidsdistinguishlivingthingsfrominanimate C materials.Itconsistsofcarbonandsomethingelse,whichincludeshydrogen,oxygen,nitrogen, sulfur,orphosphorus. Carbohydrates hesecarbohydrateshavethechemicalformulaofCnH2mOm.Itincludessimplesugars, T doublesugars,andcomplexsugars. implesugarsarecalledmonosaccharidesandtheyhavemolecularformulasthataremultiple S oftheunitCH2O.TheyallhavetheformulaC6H12O6.Glucose(whichisanexampleofthis)is thesubstrateincellularrespiration. oublesugarsarecalleddisaccharidesandtheyareformedwhentwomonosaccharidesare D joinedtogether.Maltoseisformedbyjoiningtogether2glucosemolecules,sucroseisformed byjoiningtogether1glucoseand1fructose,lactoseisformedbyjoiningtogether1glucoseand 1galactose. Studentsneedtorecognisethese: Adisaccharidecanbehydrolysed(split)toformtwomonosaccharides. omplexcarbohydratesarecalledpolysaccharides.Theyaremadeupofmanysimilar C moleculesofsinglesugarsjoinedtogethertoformalargemolecule. Fat atsorlipidscontaincarbon,hydrogen,andoxygen.Theproportionofhydrogentooxygenis F notfixed.Itcontainsmuchlessoxygeninproportiontohydrogen. atsaremostlyhydrocarbonswhichformnonpolarcovalentbonds,hencetheyaremostlyor F entirelyhydrophobic(noaffinityforwater).Afatmoleculeisformedfromtwokindsofsmaller molecules:glycerolandfattyacids.Inafatmolecule,threefattyacidsarejoinedtooneglycerol byanesterlinkage,formingatriglyceride.Mostanimalfatsaresaturated.Saturatedfatsare solidatroomtemperaturewhile“plantandfish”unsaturatedfats(AKAoils)areliquidatroom temperature,wherekinkscausedbycisdoublebondspreventthemoleculesfrompacking tightlyenoughsuchthattheyareasolidatroomtemperature. Thefunctionsoffatsarebelow: ❡Storageofenergy A gramoffatcontainsmorethantwicetheenergyofagramofpolysaccharides.Plants useoilswhendispersalandcompactstorageareimportant,suchasinseeds.Animals carryenergystoreswiththem,sotheybenefitfromhavingamorecompactfuelreservoir offat.Humansandothermammalsstorefatsaslong-termenergyreservesinadipose cellsthatswellandshrinkasfatisdepositedandwithdrawnfromstorage. ❡Cushionvitalorgans(suchaskidneys) ❡Insulation Proteins hesethingsaremadeupofthefollowingelements:Hydrogen,Oxygen,Nitrogen,Carbon,and T Sulfur. hebasicunitofproteinistheaminoacid.20naturally-occurringonesareknown.Aminoacids T arejoinedusingpeptidebonds.Short-chainproteinsarecalledpolypeptides. Proteinsfunctionfor: ❡Synthesisofnewprotoplasmforgrowthandrepairofworn-outpartsofthebody ❡Synthesisofenzymes ❡Synthesisofhormones ❡Formationofhaemoglobin ❡Formationofantibodies(todefendagainstforeignsubstances) ❡Formationofsupportstructures(eg.silk,webs,collagen,keratin) ❡Formationofstoragestructures(eg.ovalbumin,casein) ❡Membraneproteinswhichmoveacrossthephospholipidbilayer NucleicAcids ucleicacidsconsistofHydrogen,Oxygen,Nitrogen,Carbon,andPhosphorus.Nucleicacids N consistofnucleotides.Nucleicacidsarepolymers,whilenucleicacidsaremonomers. Allnucleotidesconsistof: ❡Apentosesugar ❡Anitrogenousbase ❡Aphosphategroup herearetwodifferenttypesofnucleicacids:DNA(Deoxyribonucleicacid),andRNA T (Ribonucleicacid).DNAhasadoublehelixstructurewhileRNAhasasingle-strandedstructure. DNAconsistsofnitrogenousbasesAdenine,Guanine,Cytosine,andThymine.InRNA, ThymineisreplacedbyUracil. Tests enedict’stestforreducingsugars,Iodinetestforstarch,Biurettestforprotein,Ethanol B emulsiontestforfats. Benedict’sTestinanutshell hefirst2stepsforsolidsamplesaretoreducethefoodtoatomsextractreducingsugars T fromthesolidsandtoputtheminasolvent. Steps4and5aremeanttoheatupeverythingsuchattheresultsofBenedict’stestarevisible. 1. Solidonly:Place1cm3ofthecrushedfoodsampleintoatesttube. 2. S olidonly:Add2cm3ofwaterintothetesttubetodissolvethereducingsugarsintothe sample.Decant. 3. Place2cm3ofBenedict’ssolutionintothetesttubesample,shakethoroughlytomix. 4. Placetubeinboilingwaterbathfor2-3minutes. 5. Removethetubesfromthebathafter2-3minutes. 6. Observeandrecordchangesincolourofthemixtureifany. 7. Ifthesolutionturnedfrombluetobrick-redwithprecipitate,largeamountsofreducing sugarsarepresent. Ifthesolutionturnedfrombluetoorangeoryellow,amoderateamountofreducing sugarsarepresent.Ifthesolutionturnedfrombluetogreen,traceamountsofreducing sugarsarepresent.Ifthesolutionremainedblue,reducingsugarsareabsent. Ifnon-reducingsugarsarepresent,thefollowingfollow-uptestcanbedone: 1. (Testforreducingsugars.Ifreducingsugarsareabsent:) Useafreshsamplesolutionandheatthesampleinaboilingwaterbathwith2cm3of ydrochloricacid. h 2. Neutralisewithsodiumhydrogencarbonate. 3. PerformBenedict’stestforreducingsugars. Note:Hydrochloricacidhydrolysestheglycosidicbonds. IodineTestforStarch The1ststepforsolidsamplesistogetreadythefoodforplacingiodineon. 1. Solidonly:Place1cm3ofthecrushedfoodsampleontoawhitetile. 2. A dd2dropsofiodinesolutionontothesamplesolution(liquid)/ontothecrushedfood sample.(solid) 3. Observeandrecordchangesinthecolouroftheiodinesolution 4. Ifthesolutionturnsfrombrowntoblue-black,starchispresentinthesample. Ifthesolutionremainsbrown,starchisabsentinthesample. BiuretTestforproteins Samethingforsolids,extrastepsatthebeginning. 1. Solidonly:Placethecrushedfoodintoacleantesttube,labelthetubeappropriately. 2. Solidonly:Add2cm3ofwatertothecrushedsample.Shakethoroughlytomix,decant. 3. F orsolids:Addequalvolumeofsodiumhydroxidesolutiontothesolution.Shake thoroughlytomix.Forliquids:To2cm3ofliquid,add2cm3ofsodiumhydroxide solution.Shakethoroughlytomix. 4. Addin1%coppersulfatesolution,dropbydrop.Shakethetesttubeaftereverydrop. 5. Observeandrecordchanges. 6. Ifthesolutionturnsfrombluetoviolet,proteinsarepresent. Ifthesolutionremainsblue,proteinsareabsent. EthanolEmulsionTestforlipids 1. Solidsonly:Place1cm3ofcrushedfoodintoatesttube,labelappropriately. 2. F orsolids:Add3cm3ofethanolintothesample,shakethoroughlytomixForliquids:To somedropsofliquids(usuallyoil),add1cm3ofethanol,shakethoroughlytomix. 3. Solidsonly:Recordchangesinthesolution. 4. Solidsonly:Decant1cm3ofthemixturetoanothertesttube. 5. F orsolids:Addanequalamountofwaterintotheliquidportion.Observeandrecordthe formationofawhiteemulsion.Forliquids:Add1cm3ofwatertotheliquidportion. Observeandrecordtheformationofawhiteemulsion. 6. Ifawhiteemulsionisformed,lipidsarepresent. Ifawhiteemulsionisnotformed,lipidsareabsent. TA1Preparation:AnsweringTechniques Redtextindicatesthattherewasamistake. Untitled Worksheet2-MovementofSubstances 1aii-(proteinchannel) :Facilitatesmovementofspecificmoleculesacrossthemembranebyactingasachannelor K carrierprotein. issing:“Facilitateddiffusion”,specificationofmolecules[eg.ions,smallwatersoluble M molecules] L:“Toactasanenzyme” orrectanswer:Cellrecognition,surfaceantigen,bindingsite;formshydrogenbondswithwater C tostabilisemembranestructure :Phospholipidsactasaboundarytoprotectthecellfromtheexternalenvironmentaswellas M tofacilitateexocytosis.(Thisiscorrect) 1c-Graph,y-axisisrateofuptakeofglucose,x-axisisconcentrationofglucose xplainhowtheresultsoftheinvestigationsupporttheideathatglucoseenterscellsby E faciliateddiffusion. issing:Rateofintakeincreaseswithincreasingglucoseconcentrationupuntilaconstantrate M isreached;Withpassivediffusion,itwouldcontinuerising uggestedanswer:Theconstantincreaseofrateofintakeofglucoseslowsdowntoaconstant S rateby30arbitraryunitsofglucoseconcentration.Itsupportstheideathatglucoseentersby facilitateddiffusionbecauseregulardiffusionwouldcausetheratetocontinueincreasing. Facilitateddiffusionhasalimittotherateofdiffusion. b-Nameanddescribethefunctionofthetransportproteinresponsiblefortheentryof 2 waterintotheplantcell. Missing:Thequestionwasleftblankso- (ohwell) nswer:Aquaporins,whichprovideahydrophilicchannelforwatermoleculestoentervia A osmosis. ( Aquaporinsfacilitatethemovementofwatermoleculesacrossthemembranesofanimaland plantcells.) 3aii-Statetwotypesofmaterialsthatcanbetakeninviaendocytosis Incorrect:proteins,polysaccharides Correct:chargedparticles,hydrophilicparticles bi-ExplainwhytheabsorptionofnitrateionsbytheplantsinbatchNdiffersfromthat 5 inbatchP. Missing:HowdoesbatchPtakeupions? 1ions/minerals/nitratesinbatchPareabsorbed(only)bydiffusion 2no/limited/less,energyforactiveabsorption/transport 3because(cyanide)inhibits,respiration/ATPsynthesis 4ionsinbatchNareabsorbedbyactivetransport(anddiffusion) 5(ideaof)after10hoursnoconcentrationgradientinP 6asrateofassimilation/use=rateofabsorption(soconcentrationinplantremainsconstant) 7activetransportcontinuesinNagainstaconcentrationgradient(after10hours) 8ref.toappropriatefigs(linkedtoanexplanationofdifferentabsorptionrates) Worksheet3-BiologicalMoleculesNutrients aii-Youcouldusethetesttocomparetheamountofreducingsugarintwodifferent 3 brandsofthenutritionsupplementdrinks.Suggesthowthetestcanbeusedasa semi-quantitative. Structure:Compareresultsandcolourofmixture,listcolouroutcomes,listquantitativeaction. Missing:Ifitisthesamecolour,comparethemassofprecipitateformed. aiii-Describehowthestructureofcellulosemakesitsuitableforitsfunctionincell 3 walls Missing:Longunbranchedchains Questionsdownbelow: Describethestructureoftheendoplasmicreticulum. nswer:Theroughendoplasmicreticulumhasanouterlayerthatiscoveredinribosomes.The A ribosomemembraneispartofthenuclearmembrane.Thesmoothendoplasmicreticulumisnot coveredinribisomes,andismoretubular. Describethestructureofthenucleus. nswer:Thenuclearmembranesurroundsthenucleoplasm.Thenuclearmembraneconsists A oftwomembranes.Oneofwhichiscontinuouswiththeendoplasmicreticulum.Thenuclear membraneispeforatedwithporestocontrolthemovementofsubstancesinandoutofthe nucleus. Describethefunctionofthegolgibody. nswer:Thegolgibodychemicallymodifiessubstancesmanufacturedbytheendoplasmic A reticulumandstoresandsecretesthemoutofthecellusinggolgivesicles. Describethefunctionofthegolgivesicle. nswer:Golgivesiclesareresponsiblefortransportingsubstanceschemicallymodifiedbythe A golgibodyoutofthecellviaexocytosis. Explainwhythisisknownasthefluidmosaicmodel. Answer:Thephospholipidsmovefreelywithinthebilayer Whydidthepotatogaininmass?(Itwasplacedinwater) nswer:Thepotatowasplacedinasolutionwhichhadahigherwaterpotentialthanitself. A Watertravelledfromthewater(higherconcentration)tothepotato(lowerconcentration)by osmosisthroughthepartiallypermeablecellmembranesofthepotato,downtheconcentration gradientuntilequilibriumwasreached.Thepotatocellscontainedmorewaterthanitdidbefore, thereforeitgainedinmass. Whatisthedifferencebetweenchannelandcarrierproteinsinfunction? Answer:Channelproteinscreatesahydrophilicchannelforwater-insolublemolecul Whatarethetypesofendocytosis?Describeandexplain DescribetheBiuretTest NutritioninHumans Basicoverview Therearetwotypesofnutrition:AutoprophicandHeterotrophic. nAutotrophisanorganismwhichcanproduceitsownfoodusinglight,water,carbondioxide, A orotherchemicals.Mostusephotosynthesistosynthesizefood. Heterotrophisanorganismthatisunabletoproduceorganicsubstancesfrominorganicones A anddependindirectlyordirectlyonautotrophsfornutrientsandenergy. DigestiveSystem,AlimentaryCanal,Accessoryorgans hehimandigestivesystemconsistsofthealimentarycanalandvariousaccessoryglands. T Accessoryglandsincludethesalivaryglands,pancreas,liver,andgallbladder. Mouth,BuccalCavity,SalivaryGlands hysicalandChemicaldigestionoffoodbeginsinthebuccalcavity.Teechcut,grind,andbreak P upfoodduringchewing.Thisincreasesthesurfaceareaoffoodexposedtodigestiveenzymes. hepresenceoffoodtrivggersanervousreflexwhichcausesthesalivaryglandstosecrete T salivathroughsalivaryductstothebuccalcavity.Salivacontainsamylase,mucin,buffers,and anti-bacterialagents. Salivaryamylasedigestsstarchintomaltose.TheoptimumpHforthisis7. Oesophagus hebolusispushedalongtheoesophagusbyperistalsis,notgravity.Therearerhythmicwaves T ofcontractionbysmoothmusclesinthewallsofthealimentarycanal. Stomach astricglandsinthestomachsecretesgastricjuice.Itmixesthiswiththefoodusingthe G churningactionofthesmoothmusclesinthestomachwall.Hydrochloricacidingastricjuicehas apHof2,whichkillsbacteriaanddenaturesproteinsinthefood.Pepsinworkswellinstrongly acidicenvironments.Itbreakspeptidebondsinproteinswhichproducessmallerpolypeptides. Clandpepsinarereleaseddirectlyintothelumenofthestomachsothecellsofthestomach H areprotectedfromself-digestion.Thestomachliningisfurtherprotectingbyacoatingofmucus whichissecretedbytheepithelialcells.However,theepitheliumiscontinuouslyeroded,and arereplacedregularlybymitosis. Intestinaljuicecontainspeptidase,maltase,sucrase(invertase),lactase,andlipase. Peristalsis Inperistalsis(especiallyintheoesophagus),thetwosetsofmusclesarelongitudinaland circularmuscles,whichworkantagonistically.Whenonecontracts,theotherrelaxes.Thefood issqueezedandpushedforward. !https://prod-files-secure.s3.us-west-2.amazonaws.com/94a250b5-19bc-4880-9eb3-ccfe47b69b 88/df0f75eb-e04b-446c-9d0c-f233a7f96b88/image1.png Absorption minoacidsandglucoseareabsorbedinthebloodcapillariesofthevillibydiffusiondownthe A concentrationgradients,andactivetransportagainsttheconcentrationgradient.Capillaries convergeintothehepaticportalveinwhichgoestotheliver. !https://prod-files-secure.s3.us-west-2.amazonaws.com/94a250b5-19bc-4880-9eb3-ccfe47b69b 88/e7faa236-e4c9-4392-b3cf-3ac4a3428e9a/image4.png UnknownContent-BiologyWA2 Enzymes atabolicpathways-Releasesenergybybreakingcomplexmoleculesintosimpler C compounds. Anabolicpathways-Usesenergytobuildlargercomplexmoleculesfromsmallersimplerones. nzyme-Biologicalcatalyststhataltertherateofchemicalreactionswithoutbeing E chemicallychangedattheendofareaction. Enzymeslowertheactivationenergyrequiredforreactions. ctivationenergyistheamountofenergyrequiredtobringamoleculefromitsreactantstoits A transitionstate. !https://prod-files-secure.s3.us-west-2.amazonaws.com/94a250b5-19bc-4880-9eb3-ccfe47b69b 88/39d5e997-2328-4d2d-b06f-f367e8a78ddb/image21.png nzymesarerequiredtolowertheactivationenergyrequiredforreactionsbecauseheatingto E dosowoulddenatureproteinsandisnotsuitablefororganisms. ockandkeyhypothesis:Reactioncatalysedbyeachenzymeisveryspecific.Theenzyme L willbindtoasubstratewithacomplementaryshapewhichfitsintotheactivesiteoftheenzyme toformanenzyme-substratecomplex.Whilebound,thecatalyticactionoftheenzyme convertsthesubstrateintotheproducts. Reasonofspecificity-Three-dimensionalshape,consequenceofaminoacidsequence nzymeactivitycanbeinvestigatedeitherbymeasuringtheformationofproductsorthe E disappearanceofsubstrates. Nutritioninhumans Ingestion>Digestion>Absorption>Assimilation>Egestion outh-Physicalandchemicaldigestionoccurinthebuccalcavity.Presenceoffoodtriggersa M nervousreflexwhichsecretessalivathroughsalivaryductstothebuccalcavity. wallowing-Tonguerollsfoodintoabolus,andpushesitintothebuccalcavityandpharynx. S Glottis(tracheaopening)isblockedbyepiglottisflap. Smallintestineparts-Duodenum>Ileum>Jejunum uodenum-Secretesintestinaljuiceintolumencontainingpeptidase,maltase,sucrase, D lactase,lipase.Mostdigestioniscompletedhere. Ileum-Absorptionofdigestedfood Jejunum-Absorptionofdigestedfood bsorptiondetails-Aminoacidsandglucoseareabsorbedintobloodcapillariesbydiffusion A andactivetransport.Capillariesconvergeintolargerbloodvesselsandthenthehepaticportal veinleadingtotheliver. G lycerolandfattyacidsareabsorbedbydiffusiononlyintotheepithelialcellsand recombinedintofatmolecules,thenmixedwithcholesterolandspecialproteinstoform chylomicrons,thentransportedintolactealsofeachvillus. mallintestineisadaptedforabsorptionwithalargesurfacearea,netofbloodcapillariesand S lactealscausingasteepconcentrationgradient,one-cellthickepithelium,andmany mitochondriainepithelialcellstofacilitateactivetransport. Pancreas-Producesalkalinepancreaticjuicecontainingtrypsin(protease),amylase,lipase. Liver -Secretesinsulintostimulateconversionofexcessglucosetoglycogenifblood 1 concentrationishigh ecretesglucagontostimulateconversionofexcessglycogentoglucoseifblood S concentrationislow Bloodglucoseconcentration=90mg/100ml=0.9g/dm3 2-Secretesbilethatisstoredinthegallbladder. 3-Breaksdownhormonesafteruse -Metabolisesexcessaminoacidsbydeaminationtoeventuallybeconvertedintoureaand 4 glycogen. 5-Breaksdownalcoholbyoxidationtoacetaldehyde Self-test.Boldedareunknown 5functionsoftheliver? . 1 loodglucoseconcentrationregulation B 2. Hormoneremoval 3. Productionofbilefordigestion 4. Oxidationofalcohol 5. Deaminationofexcessaminoacids Howcanyoumeasuretherateofenzymeactivitywithanexperiment?Therearetwoways. Howareaminoacidsandglucoseandfatmoleculesabsorbedinthesmallintestine? Transport Consistsofbloodandlymphaticsystem Doublecirculation,pulmonaryandsystemic Lymphaticsystemisone-wayfrominterstitialfluidtocardiovascularsystem B loodconsistsofbloodplasma(55%),redbloodcells,whitebloodcells,and platelets. B loodplasmacontainsinorganicions/salts,plasmaproteins,organicnutrients, nitrogenouswastes,hormones,dissolvedgases Redbloodcells(erythrocytes)producedbybonemarrow,containshaemoglobin P roteinmoleculehaemoglobinconsistsoffoursubunits,whicheachconsistofglobular proteinsattachedtohaemunit(whichthencomprisesofporphyrinringcontainingiron atom) A daptationsoferythrocytes:Biconcavecircular,haemoglobin,nonucleus,flexible plasmamembrane,smallsize Nonucleusallowsredbloodcelltocarrymorehaemoglobin(notoxygen) Acclimatisation Whitebloodcells(leucocytes)havetwotypeslymphocytesandphagocytes L ymphocytes-Largeroundednucleus,limitedmovement,doesn’tsqueezethrough capillarywalls,producesantibodiesinresponsetoforeignproteinsantigensfrom pathogens. Antibodiesdoesthefollowing: Causebacteriatoagglutinatetofacilitatephagocytosis/ingestionbyphagocytes Neutralisestoxinsfrombacteria Rupturesbacteriacellmembrane P hagocytes-beanshaped,capableofamoeboidmovementandthroughcapillarywalls toinfectedareas,shorterlifespanthanlymphocytes U ndergoesphagocytosistoingestforeignparticles,engulfsittoformvesicle,fusedwith lysosomecontaininghydrolyticenzymestodigestthebacteria B loodplatelets(thrombocytes)initiatebloodclottingtopreventexcessivelossofblood andentryofharmfulbacteria P lateletsreleaseenzymethrombin,catalysesconversionofsolubleproteinfibrinogen toinsolublefibrinthreads Fibrinthreadsentanglebloodcells,wholemassformsaclotorscab Bloodgroupnumberrepresentstheantigensithas.(GroupOmeansnoantigens) Antigensandantibodiesofthesametypeagglutinates(Aagglutinateswitha) Antigensmarkedwithcapitalletters,antibodiesmarkedwithsmallletters ABistheuniversalacceptor,Oistheuniversaldonor Tissuerejectionoccurswhentransplantedorganisnotcompatiblewithpatient’soriginal Generalbloodflow:Artery-Arteriole-Capillary-Venule-Vein A rterieshasthickmuscular,andelasticwalls;andasmallerlumenthanveins;blood flowsunderhighpressureinpulses Thickmuscularwallsconstrictsanddilatesarteriestomaintainpressure Elasticwallswithstandshighpressurebyventricularcontractionsofheart V einshavelessmuscularandelasticwalls,largerlumenthanarteries,presenceof semi-lunarvalves.Bloodflowsunderlowerpressure Semi-lunarvalvespreventbackflowofbloodwhenclosed Capillariesaremicroscopicbloodvessels,wallsaresinglelayerofcells(endothelium) P artiallypermeableorone-cellthickendotheliumenablesquickdiffusionofsmaller substances H ighlybranchednetworkofcapillariesprovideslargesurfaceareaforexchangeof substances,reducesbloodpressuretoslowdownbloodflowforexchangeof substances Plasmaforcedoutofarterialendofcapillarytoformtissuefluid Tissuefluidisamediumfortransferofsubstancesbetweencapillariesandcell Dissolvedfoodandoxygendiffusesfromcapillariesintotissuefluidthenintocells Wasteproductsgotheotherway T issuefluidreturnstocirculatorysystembyvenousendofcapillarynetworkand lymphaticcapillaries S wellingoforgansandtissuesoccurswhenaccumulatedtissuefluidisnotreturnedto blood Advantagesofdoublecirculation: Bloodenterslungsatlowerpressure,sufficienttimeforbloodtobeoxygenatedwell Bloodpumpedtobodytissuesathigherpressurebyheart,distributesoxygentothem faster H earthas2upperchambersatriaand2largerlowerchambersventricles;rightside completelyseparatedfromleftbyseptumtopreventmixingofoxygenatedand deoxygenatedblood A triahasthinnerwallsthanventriclesbecausedistancebetweenatriaandventriclesare short L eftventriclehasthickerwallsthanrightventriclebecausesystemicvspulmonary circulation P ath:Venacava-Rightatria-Rightventricle-Pulmonaryartery-Lungs-Pulmonary vein-Leftatria-Leftventricle-Aorta-Body D iastole(0.4s):Allmusclesrelaxed,bloodflowsintotheheart,oxygenatedblood entersleftatrium,deoxygenatedbloodentersrightatrium,bloodflowspassivelyinto ventricleswhenbicuspidandtricuspidvalveareopen A trialSystole(0.15s):Musclesofatriacontract,musclesofventriclesremainrelaxed, bloodpumpedfromventriclestoatria,aorticandpulmonaryvalvesremainclosed V entricularSystole(0.3s):Musclesofventriclescontract,musclesofatriabeginto relax,steepincreaseofpressureclosesbicuspidandtricuspidvalvesforcefully causing“Lub”,aorticandpulmonaryvalvesforcedopen,bloodforcedintorespective vessels(aortaandpulmonaryartery) V entricularDiastole:Ventriclesstarttorelax,pressurefalls,highpressurefromaorta andpulmonaryarteriescausesbloodtoflowbacktowardsventricles,closesaorticand pulmonaryvalves,preventsbackflowofblood,causes“Dub”. A ccumulationofcholesterolandpolysaturatedfatsaredepositedoninnerwallof coronaryarteriesduringatherosclerosis,lumennarrows,bloodpressureincreases Respiration R espirationoccursinmitochondriaofalllivingcells.Eachstepiscontrolledbyenzymes. Breakdownoffoodsubstancesthroughoxidationinlivingcellswiththereleaseof energy. Pointofrateofphotosynthesisbeingequaltorateofrespirationinplantsis ➡️ compensationpoint. Aerobicrespiration:C6H12O6+6O2 6CO2+6H2O|Largeamountofenergy ➡️ released Anaerobicrespiration:C6H12O6 2CO2+2C2H5OH|Relativelysmallamt.ofenergy ➡️ released,ethanolonright,anaerobicrespirationinyeast Anaerobicrespiration:C6H12O6 2C3H6O3|Relativelysmallamt.ofenergy released,lacticacid,anaerobicrespirationinhumans Totalenergyneeded=Aerobicrespirationenergy+Anaerobicrespirationenergy Oxygendebtistheamountofoxygenneededtobreakdownalllacticacidaccumulated inmusclesduringanaerobicrespiration. Oxygendebtisincurredduetoinsufficientoxygentomeetdemandsofmuscular contraction Lacticacidisremovedfrommusclesandtransportedtoliver,someisoxidisedtorelease energytoconvertremaininglacticacidintoglucosethenglycogenasstorage. Passageofair:Mouth/Nostrils-Pharynx-Larynx-Trachea-Bronchi-Bronchioles- Alveoli Hairsandmucousmembranetrapdustandbacteriainairinnostrils Sensorycellsinmucousmembranedetectharmfulchemicals Glandcellsintracheasecretemucustotrapdustandbacteria Ciliatedcellsbearciliatosweeptrappeddustandbacteriaupwardsintothepharynx, thenswallowedintotheoesophagus.(SHITEihavephlegmnow) Thinlayeroflubricatingfluidallowspleura(amembrane)toglideoveroneanothereasily whenlungscontractorexpandduringbreathing. TotalvolumeofairinlungsconsistsofInspiratoryreservevolume(maxinhalelimit), Tidalvolume(regularbreathinglimit),Expiratoryreservevolume(maxexhalelimit), Residualvolume(airthatcannotbeexhaled). Oxygendissolvesinmoistureliningalveolarwalls,diffusesthroughalveolarwallsinto bloodcapillaries. Oxygenentersbloodplasma,combineschemicallywithhaemoglobintoform oxyhaemoglobin Alveolicontaincollagenandelasticfibersthatallowalveolitostretchduringinhalationto fillitwithair Concentrationgradientofgasesismaintainedbycontinuousflowofbloodand constantbreathingofair. Alveoliadaptationsinclude(below) umerousalveoli,largesurfacearea N One-cellthickalveoli,shortdiffusiondistance Thinfilmofmoistureonsurfaceofalveolus,allowsoxygentodissolve Continuousbloodflow,maintainsconcentrationgradient InINHALATION:(Torememberantagonsticmotion,respectivemusclerelaxes-External relaxesuponexpiration) Externalintercostalmusclescontract,shorten Internalintercostalmusclesrelax,lengthen Ribsandsternummoveupwardsandoutwards Diaphragmcontractsandflattens Volumeoflungsincreases,pressuredecreases InEXHALATION: Externalintercostalmusclesrelax,lengthen Internalintercostalmusclescontract,shorten Ribsandsternummovedownwardsandinwards Diaphragmrelaxesandarchesoutwards Volumeoflungsdecreases,pressureincreases Atmosphericpressureishigher/lowerthanpressureinlungs,airpassivelyflowsin/outof lungs BreathingstimulusistheaccumulationofcarbondioxidecausingalowerpH. Lackofoxygenisnotabreathingstimulus Nicotine-addictive,increasesheartrate,bloodpressure,andbloodclotsinblood vessels Carbonmonoxide-deadly,combinesirreversiblywithhaemoglobintoform carboxyhaemoglobin,reducesamountofhaemoglobininblood,increasesbloodclotsin bloodvessels Tar-cancerous,blocksairsacs,paralysescillialiningairpassage,preventsremovalof mucus . 1 Defineoxygendebt 2. Howisoxygendebtmanaged 3. Statethepassageofairintotherespiratorysystem 4. Statethepassageofoxygenintothebloodstream 5. Statetheadaptationsofthenoseandtracheatoremovebacteriaordust. 6. Listtheactionofintercostalmuscles,ribsandsternum,anddiaphragmininspirationand expirationaswellasvolumeandpressureoflungs. . 7 Statetheactionofbreathing 8. Statewhysmokingbad Excretion M etabolicwasteproductsandtoxinsareremovedinexcretion Includesurea,carbo=ndioxide,bilepigments,water,oxygen(inphotosynthesis) Preventsaccumulationofmetabolicwaste,whichcanalterpH/waterpotentialofbody fluidsorpoisonenzymesystems Carbondioxideexcretedasgasinexhaledair M ineralsalts,nitrogenouswasteproducts(urea,uricacid)andcreatinine(frommuscle tissuebreakdown)excretedasurineandsweat(insmallerquantities) Excesswaterexcretedasmainconstituentofurineandsweatandasvapourinexhaled air Bilepigmentsexcretedasconstituentoffaecesthroughintestines Excretionistheremovalofmetabolicwasteproducts Egestionistheremovalofundigestedwaste Oxygenisanexcretoryproduct.Photosynthesisisametabolicprocess(anabolic). Urinarysystemconsistsof: Pairofkidneys,renalarteryandrenalvein,pairofureters,bladder,urethra.(renal referstokidney) Renalarterieshasmoreureaandlesscarbondioxidethanrenalveins Kidneylocatedinabdominalcavityattachedtothedorsalwallbehindtheliver. Nephronisfoundinthecortexoftherenalpyramidinakidney IncludesBowman’scapsule,Proximal(first)convolutedtubule,loopofHenle,Distal (second)convolutedtubule Severalnephronsopenintothecollectingductwhichisatube Bloodenterskidneybyrenalarteryandbranchesintoglomerulus(amassofcapillaries inBowman’scapsule) Afferentarterioleiswiderthanefferentarteriole. Heartanddiameterdifferenceofafferentandefferentarteriolescreatehighpressurein glomerulus ForcespartofbloodplasmathroughthecapillarywallsintoBowman’scapsule Largermolecules(redbloodcells,whitebloodcells,platelets,fats,proteins)areretained Filtratemovesofglomerulustothethreeotherparts. Usefulmaterialsarefilteredbackbycapillariessurroundingthetubules.(Glucose,amino acids,mostmineralsalts) Anti-diuretichormone(ADH,vasopressin)actionregulatestheconcentrationsofwater andvarioussolutestomaintainaconstantwaterpotentialinthebody. LessADHmeansmoreurine.MoreADHmeanslessurine.“Anti”meansLessand “Diuretic”meansurine. Increasespermeabilityofthedistalconvolutedtubulesandcollectingduct HomeostasisandHormonalcontrol Homeostasisismaintenanceofconstantinternalenvironment Receptor,controlcentre,effector N egativefeedbackcausesbodytobringoppositecounterchangetochangesdetectedto restoresystemintoitssteadystate. Bloodglucoselevelregulation-Liver(normalis90mg/100cm3ofblood) Hyperglycemia-Temporaryincreaseinbloodglucoseconcentration IsletsofLangerhansβ-cellsinpancreasarestimulated,releaseshormoneinsulin Insulindiffusesfrompancreasinto(surrounding)bloodcapillaries,transportedintoliver andmuscles Promotesconversionofexcessglucosetoglycogenforstorage(1) Causesincreasedcellularrespiration(2) Increasespermeabilityofcellmembranetoglucose(3) Remaininginsulinsenttolivertobedestroyed(deamination),removedbykidneys. Hypoglycemia-Temporarydecreaseinbloodglucoseconcentration IsletsofLangerhansα-cellsinpancreasarestimulated,releaseshormoneglucagon Osmoregulation-Kidneys(regulatingwaterpotentialinblood) C ontrolledbyADH(Antidiuretichormone),producedbyhypothalamusinbrain, releasedbypituitarygland,increaseswaterreabsorptionatkidneynephrons P reventsredbloodcellsfrombeingaffectedbywaterpotentialchangesbylysisor crenation Thermoregulation-Skin C ontrolledbysudoriferousglandsandvasodilationduringheatgain,increasein metabolicrateandvasoconstrictionduringheatloss,allbyhypothalamus Arterio-venousshuntvesselcanconstrictordilate D uringheatgain,arterio-venousshuntvesselconstricts,bloodisdirectedtosurface capillaries,moreheatlostbyradiation D uringheatloss,arterio-venousshuntvesseldilates,lessbloodisdirectedtosurface capillaries,lessheatlostbyradiation Coordinationandresponseinvolvesnervoussystemandendocrinesystem E ndocrinesystemcontrolsbodyactivitybyreleasingchemicalmessengers(aka hormones)intoblood H ormone-Chemicalsubstanceproducedbyanendocrineglandcarriedbyblood, whichalterstheactivityofoneormorespecifictargetorgansandisthendestroyedby theliver. Hormonesdiffusedirectlyintosurroundingblood,henceendocrineglandsareductless Endocrineglandscontainanextensivenetworkofbloodvessels Pituitarygland-“controller”ofotherendocrineglands,secretesADH Hypothalamus-Regulatesthesecretionofpituitaryhormones T hyroidgland-Secretesthyroxine,whichcontrolsrateofmetabolismandinfluences physicaldevelopment Adrenalgland-Secretesadrenaline,preparesbodyfor“fight-or-flight” Pancreas-IsletsofLangerhanssecreteinsulinandglucagon Testes-Secretestestosterone Ovaries-Secretesestrogen Type1diabetesisinsufficientinsulin,Type2diabetesisinsulinresistance. Humanskinfunctionsbelow: ○ Thermoregulation ○ Thermalinsulation ○ Protectionfromphysicaldamage,dehydration,UVrays ○ Energyreservesfromadiposetissue ○ Receptionofexternalstimuli ○ Vitaminproduction ○ (minor)nitrogenousexcretion Consistsofepidermisanddermis Nervoussystem Actionscanbevoluntaryorinvoluntary NervoussystemconsistsofCentralNervousSystemandPeripheralNervousSystem CNSconsistsofbrainandspinalcord P NSconsistsofnerves,ganglia,senseorgans,formsconnectionbetweenorgansand CNS N erveimpulsesaretransmittedalongneuronsfromorgansthatreceivestimuliat receptorstoorgansthateffectchangeateffectors. Example:Nerveimpulsetransmittedfrompainreceptortospinalcordthentomuscle Cellbodyofneuroncontainsnucleusandcytoplasm S ensoryneuron(receptor)-transmitnerveimpulsefromsenseorganstoCNS(optic nerve) R elayneuron(intermediate)-foundwithinCNS,receiveimpulsesfromsensoryorother intermediateneurons,relayingtootherintermediateormotorneurons. Motorneuron(effector)-transmitnerveimpulsefromCNStoeffectors Partsofaneuron(below) Dendron-nervefibresthattransmitnerveimpulsestowardsthecellbody Dendrite-receiverofnerveimpulsesfromotherneurons Axon-nervefibrethatcarryimpulsesawayfromthecellbody Myelinsheath-layeroffattysubstancethatshieldsandinsulatesthenervefibre N odeofRanvier-regionwheremyelinsheathisabsent,speedinguptransmissionby allowingimpulsestojumpfromnodetonode Axonterminals-transmitnerveimpulsestotheeffector Motorendplate-junctionbetweenaxonterminalsandmusclefibre (Ifinitalics,“axonterminals”werepreviouslymistakenfordendrites) Motorneuronhasterminalcellbody,numerousshortnervefibresleadintoit Sensoryneuronhasnon-terminalcellbody,singlelongnervefibreleadsintoit N ervefibreisastrandofcytoplasmextendingfromcellbody,specialisedintransmitting nerveimpulses Anerveisabundleofnervefibres,enclosedinasheathofconnectivetissue S ensorynervefibresconductnerveimpulsesfromsenseorgans,whilemotornerve fibresconductnerveimpulsestoeffectors Spinalnervescontainbothfibres,calledmixedfibres Asynapseisajunctionbetweentwoneuronsorbetweenaneuronandaneffector N euronsrarelycontacteachother,impulsesdonottransmitdirectlyfromneuronto neuron N erveimpulsesaretransmittedelectricallythroughneurons,chemicallyacross synapses Ensuresone-directionalflowofimpulses Impulsesarecarriedacrosssynapsesvianeurotransmitters(aka.Chemicalmeans) S pinalcordpassesthroughvertebralcolumn,whichprotectsspinalcord(veryimportant, break=paralysedfromabovetheheaddown) R elaysimpulsesinandoutatanypointalongthecord,upanddownthebody,toand fromthebrain Spinalcordcontainscellbodies,synapses,andrelayneurons,whichisgreymatter. Relaysfollowing: ○ Sensoryneurontobrain ○ Braintomotorneuron ○ Sensoryneurontomotorneuron(reflexaction) Outerpartofthecordcontainslongitudinally-runningfibres,calledwhitematter. S ensoryfibresenterthroughthedorsalroot,motorfibresleavethroughtheventral root. C ellbodiesofsensoryfibresaresi