What characteristic of restriction enzymes limits their effectiveness in genetic manipulation?
Understand the Problem
The question is asking about a specific characteristic of restriction enzymes that hinders their use in genetic manipulation. It is focused on understanding the properties of these enzymes in a biological context.
Answer
Restriction enzymes require specific short DNA sequences to work, limiting their effectiveness.
The specific characteristic of restriction enzymes that limits their effectiveness in genetic manipulation is their requirement for recognizing and cleaving at specific short DNA sequences.
Answer for screen readers
The specific characteristic of restriction enzymes that limits their effectiveness in genetic manipulation is their requirement for recognizing and cleaving at specific short DNA sequences.
More Information
Restriction enzymes are vital in molecular biology for their ability to cut DNA at specific loci, known as restriction sites. However, their specificity also presents a limitation, as their utility is confined to instances where their target sequences exist in the DNA being manipulated. This limits their use in editing complex genomes where such sequences might not be present or are too infrequent.
Tips
A common mistake is assuming restriction enzymes can cut anywhere in the DNA sequence, which isn't true. They can only act at specific site matches.
Sources
- Restriction Enzyme - an overview | ScienceDirect Topics - sciencedirect.com
- How to Use Restriction Enzymes: A Resource Guide - promega.com
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