BCH3004- Photosynthesis

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Questions and Answers

What is the name of the specific anatomical arrangement of cells in C4 plants, and how are the cells arranged?

Kranz anatomy; mesophyll cells are loosely arranged between the bundle sheath and the leaf surface.

In C4 plants, what are the two main types of malic enzyme and where does decarboxylation occur?

NADP-malic enzyme and NAD-malic enzyme; Decarboxylation occurs in the bundle sheath chloroplasts with NADP-malic enzyme and in the mitochondria of bundle sheath cells with NAD-malic enzyme.

Why do CAM plants open their stomata at night, and what do they store as a result?

To minimize water loss due to evaporation by using the cooler, more humid night air to take in CO2; organic acids.

During what part of the day in CAM plants is the stored CO2 released, and where does the release of the CO2 occur?

<p>Day; chloroplast.</p> Signup and view all the answers

How do CAM plants solve the problem of water loss during photosynthesis?

<p>By opening their stomata only during the night when it is cool and there is higher air humidity.</p> Signup and view all the answers

What is the primary function of chlorophyll in photosynthesis?

<p>To absorb light energy.</p> Signup and view all the answers

What are the two main types of reactions in photosynthesis?

<p>Light-dependent and light-independent reactions.</p> Signup and view all the answers

What is the approximate ratio of chlorophyll a to chlorophyll b in plants?

<p>3:1.</p> Signup and view all the answers

In which part of the chloroplast do light-dependent reactions occur?

<p>Thylakoids.</p> Signup and view all the answers

What is the primary outcome of the Calvin cycle?

<p>Carbon reduction.</p> Signup and view all the answers

What is the reaction center of Photosystem I (PSI)?

<p>P700</p> Signup and view all the answers

What is the structural difference between chlorophyll a and chlorophyll b?

<p>Chlorophyll a has a methyl group, whereas chlorophyll b has a formyl group.</p> Signup and view all the answers

What is the effect of Emerson enhancement?

<p>The enhancement of photosynthesis when exposed to two wavelengths rather than one.</p> Signup and view all the answers

What is the primary function of the light-harvesting complex (LHC) in photosynthesis?

<p>To absorb light energy and funnel it into the reaction center.</p> Signup and view all the answers

What is the immediate result when a chlorophyll molecule absorbs a photon of light?

<p>An electron within the molecule is energized and moves to a higher energy orbital.</p> Signup and view all the answers

What is the reaction centre of Photosystem II and what is it's absorption peak in nm?

<p>P680 with absorption peak at 680 nm.</p> Signup and view all the answers

What is the name of the process where water is split, and what are the products of this reaction?

<p>Water photolysis; oxygen, hydrogen ions, and electrons.</p> Signup and view all the answers

Where in the chloroplast do the events of Photosystem II take place?

<p>Thylakoid membranes.</p> Signup and view all the answers

What is the fate of an excited electron in a chlorophyll molecule when it returns to its ground state?

<p>The excess energy is released as heat.</p> Signup and view all the answers

What is the role of the oxygen-evolving complex (OEC) in photosynthesis?

<p>Oxidation of water, also called water photolysis, into oxygen, hydrogen ions and electrons.</p> Signup and view all the answers

What contributes to the transmembrane chemiosmotic potential that drives ATP synthesis in photophosphorylation?

<p>Hydrogen ions (protons) produced from the water photolysis.</p> Signup and view all the answers

What is the term for the process where light energy is used to produce ATP?

<p>Photophosphorylation.</p> Signup and view all the answers

What is generated across the thylakoid membrane as a result of the hydrogen ion accumulation and what does this lead to?

<p>A proton motive force; ATP synthesis.</p> Signup and view all the answers

What is the primary role of Photosystem I in cyclic photophosphorylation?

<p>To cycle electrons back from ferredoxin to the cytochrome complex.</p> Signup and view all the answers

What are two key products that are NOT generated during cyclic photophosphorylation that are in non cyclic?

<p>NADPH and oxygen.</p> Signup and view all the answers

What is the primary role of the accumulation of NADPH in the chloroplast relating to light reactions?

<p>It stimulates a shift from non-cyclic to cyclic electron flow.</p> Signup and view all the answers

Besides oxygen, what are two compounds produced by non-cyclic photophosphorylation?

<p>NADPH and ATP.</p> Signup and view all the answers

Write the overall balanced equation for photosynthesis.

<p>6CO2 + 12H2O -&gt; C6H12O6 + 6H2O + 6O2</p> Signup and view all the answers

Which enzyme catalyzes the attachment of $CO_2$ to RuBP during carbon fixation?

<p>RuBP carboxylase (rubisco).</p> Signup and view all the answers

What unstable six-carbon compound is created after $CO_2$ attaches to RuBP?

<p>An unstable six-carbon compound that immediately splits.</p> Signup and view all the answers

What is the three carbon molecule that is produced as a result of the split of the unstable 6-carbon molecule?

<p>3-phosphoglycerate.</p> Signup and view all the answers

In the reduction phase, what molecule is formed when 1,3-bisphosphoglycerate is reduced by NADPH?

<p>Glyceraldehyde 3-phosphate (G3P) also known as PGAL.</p> Signup and view all the answers

How many G3P molecules are used to synthesize one molecule of glucose?

<p>Two</p> Signup and view all the answers

How many ATP molecules are required for net synthesis of one G3P molecule in the Calvin cycle?

<p>9</p> Signup and view all the answers

Besides ATP, what other molecule donates electrons to the Calvin cycle during the synthesis of one G3P?

<p>NADPH</p> Signup and view all the answers

How many times must the Calvin cycle occur to produce one molecule of glucose?

<p>Two</p> Signup and view all the answers

What environmental condition do many C3 plants thrive in regarding temperature and water?

<p>Cool and wet</p> Signup and view all the answers

What process is induced in C3 plants when their stomata close, reducing CO2 levels?

<p>Photorespiration</p> Signup and view all the answers

Other than the loss of fixed carbon, why does photorespiration reduce photosynthetic output?

<p>It consumes ATP</p> Signup and view all the answers

Which major alteration in carbon fixation is performed by C4 plants that allows them to avoid photorespiration in hot and arid climates?

<p>Hatch-Slack pathway</p> Signup and view all the answers

What is the initial carbon fixation product in C4 plants?

<p>A four-carbon compound</p> Signup and view all the answers

Name one of the two specialized cell types in the leaves of C4 plants where photosynthesis takes place.

<p>Bundle sheath cells</p> Signup and view all the answers

In addition to the bundle sheath cells, what other cell type in C4 plant leaves perform photosynthesis?

<p>Mesophyll cells</p> Signup and view all the answers

Flashcards

Photosynthesis

The process by which plants convert light energy into chemical energy (glucose) using chlorophyll and other pigments.

Light Reaction

The first stage of photosynthesis, where light energy is captured by chlorophyll and converted into chemical energy in the form of ATP and NADPH.

Dark Reaction (Calvin Cycle)

The second stage of photosynthesis, where carbon dioxide is converted into glucose using the energy from ATP and NADPH.

Chlorophyll

The main pigment in plants that absorbs light energy, primarily in the blue and red wavelengths.

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Accessory Pigments

A group of pigments that absorb light energy and transfer it to chlorophyll a, which is the primary pigment responsible for photosynthesis.

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Photophosphorylation

A process that occurs in the thylakoid membranes of chloroplasts, where light energy is used to create a proton gradient, which drives the production of ATP.

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Carbon Reduction (Calvin Cycle)

The process by which carbon dioxide is converted into glucose using the energy from ATP and NADPH in a series of enzymatic reactions.

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Photorespiration

A process that occurs in plants when oxygen is present, where the enzyme rubisco reacts with oxygen instead of carbon dioxide, leading to a loss of energy and reduced glucose production.

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Photosystem II (PSII)

A specialized complex within chloroplasts responsible for capturing light energy and converting it into chemical energy.

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P680

The reaction center of Photosystem II, with a peak absorption wavelength of 680 nm.

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Water Photolysis

A process that occurs in the thylakoid membranes of chloroplasts, where water is split, releasing oxygen and hydrogen ions.

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Light Harvesting Complex (LHC)

The complex of proteins and pigments that captures light energy in Photosystem II.

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Reaction Center

The specialized molecule at the core of the LHC, where energy is transferred and photosynthesis begins.

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Antenna Pigments

The protein-bound pigment molecules within the LHC, absorbing light energy and transferring it to the reaction center.

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Electron Excitation

The process where the absorbed light energy excites an electron in a chlorophyll molecule, raising it to a higher energy level.

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Excited State

The unstable state of a chlorophyll molecule after absorbing light energy, where the electron is at a higher energy level.

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Energy Release

The process where the excited electron in a chlorophyll molecule returns to its ground state, releasing energy.

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Cyclic Photophosphorylation

A type of photosynthesis where the electrons flow in a cycle from photosystem I back to the cytochrome complex, generating ATP but not NADPH or oxygen.

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Photosystem I in Cyclic Photophosphorylation

Cyclic photophosphorylation uses only photosystem I.

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Electron Flow in Cyclic Photophosphorylation

The electron from ferredoxin returns to the cytochrome complex in cyclic photophosphorylation.

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No NADPH or Oxygen in Cyclic Photophosphorylation

Cyclic photophosphorylation does not produce NADPH or oxygen.

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ATP Production in Cyclic Photophosphorylation

Cyclic photophosphorylation generates ATP.

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Products of Non-cyclic Photophosphorylation

Non-cyclic photophosphorylation produces NADPH, ATP, and oxygen.

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ATP Demand in Calvin Cycle

The Calvin cycle uses more ATP than NADPH.

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NADPH Regulation of Photosynthesis

The concentration of NADPH in the chloroplast influences the choice between non-cyclic and cyclic photophosphorylation.

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Shift to Cyclic Photophosphorylation

An increase in NADPH stimulates a shift from non-cyclic to cyclic electron flow.

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Role of Cyclic Photophosphorylation

Cyclic photophosphorylation helps to balance ATP and NADPH production, ensuring proper functioning of the Calvin cycle.

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Kranz Anatomy

The arrangement of cells in C4 plants where bundle sheath cells are surrounded by mesophyll cells, facilitating efficient carbon dioxide fixation.

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C4 Pathway

A photosynthetic pathway that uses an initial carbon fixation step to produce 4-carbon organic acids, minimizing photorespiration and increasing efficiency in hot, dry environments.

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Pyruvate-phosphate dikinase

An enzyme that catalyzes the conversion of pyruvate to phosphoenolpyruvate in the C4 pathway, providing the substrate for initial CO2 fixation.

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CAM (Crassulacean Acid Metabolism)

A metabolic pathway found in plants that fix carbon dioxide at night when stomata are open, storing it as organic acids and releasing it during the day for photosynthesis.

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Malic Enzyme

An enzyme that decarboxylates malic acid, releasing carbon dioxide for the Calvin cycle, and is involved in both C4 and CAM pathways.

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What is Rubisco?

C3 plants use this enzyme to fix carbon dioxide, but under certain conditions, it can also bind to oxygen instead.

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What happens when Rubisco binds to oxygen?

When Rubisco binds to oxygen instead of carbon dioxide, it leads to a process called photorespiration, resulting in a loss of energy and decreased photosynthetic efficiency.

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Why does photorespiration occur in C3 plants?

Photorespiration occurs when stomata are closed to conserve water, leading to lower carbon dioxide levels inside the leaf. This forces Rubisco to bind to oxygen instead of carbon dioxide.

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Describe the photorespiration process in C3 plants.

This process involves a series of reactions that convert the compound produced from Rubisco's reaction with oxygen (phosphoglycolate) into glycine, which then releases carbon dioxide. This process consumes ATP and produces no sugar, decreasing the plant's photosynthetic output.

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What is the C4 pathway?

C4 plants have evolved a mechanism to minimize photorespiration, using an alternative carbon fixation pathway that produces a four-carbon compound initially, before entering the Calvin cycle. This process involves specialized cells and enzymes.

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What are the bundle sheath cells in C4 plants?

In C4 plants, these cells surround the veins of the leaf and are specialized for the Calvin cycle. They receive the four-carbon compound produced in the initial fixation step.

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What are the mesophyll cells in C4 plants?

These cells are specialized for the initial carbon fixation step in C4 plants, producing a four-carbon compound that then gets transported to the bundle sheath cells.

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What are C4 Plants?

These plants are named for the four-carbon compound produced in the initial carbon fixation step instead of the usual three-carbon compound produced by C3 plants. They are well-adapted to hot and arid conditions.

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What is the CAM pathway?

This process involves a different carbon fixation pathway utilized by some plants, especially those adapted to arid environments. It involves storing carbon dioxide during the night and releasing it during the day to minimize water loss during photosynthesis.

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What is C3 photosynthesis?

This is the most common type of photosynthesis, employed by most plants, where carbon dioxide is initially fixed into a three-carbon compound. It is efficient in cool and wet climates but less so in hot and dry conditions.

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Study Notes

Photosynthesis Overview

  • Photosynthesis is a crucial biochemical process
  • It's the primary source of carbon in plants
  • Drought stress reduces photosynthetic activity
  • This is due to decreased CO2 diffusion to chloroplasts

Light Reactions

  • Light reaction involves chlorophyll and other pigments
  • The Emerson effect impacts photosynthesis
  • Energy and photosynthetic phosphorylation occur
  • Dark reaction, Calvin cycle, and carbon reduction occur

Other Key Processes

  • C3, C4, and CAM cycles are involved in carbon reduction
  • The process of photorespiration also takes place
  • Different types of cyanobacteria exhibit complex morphologies
  • Light energy content depends upon wavelength
  • Chlorophyll a and b have specific absorption peaks

Types of Chlorophyll

  • Chlorophyll a is most common in plants
  • Chlorophyll b, c, d, and f have other functionalities
  • Protochlorophyll is found in seedlings in the dark
  • Bacteriochlorophyll is seen in various bacteria
  • Chlorobium has associated chlorophylls

Stages of Photosynthesis

  • Light reaction occurs in thylakoids
  • Light-independent reaction (Calvin cycle) happens in stroma

Light Reaction Details

  • Light energizes electrons in thylakoid membranes
  • Electron transport chain occurs
  • ATP and NADPH are synthesized

Photosystems I and II

  • Two photosystems (PSI and PSII) collaborate
  • Photosystem I has absorption peaks at 700 nm
  • Photosystem II has the absorption peak at 680 nm
  • Oxygen evolution complex is involved
  • Light-harvesting complexes (LHC) absorb light energy

Calvin Cycle Phases

  • Phase 1: Carbon fixation
  • Phase 2: Reduction (involves NADPH and ATP)
  • Phase 3: Regeneration of RuBP (ribulose bisphosphate)

Photophosphorylation

  • Proton motive force is crucial in generating ATP
  • Two types of photophosphorylation exist
    • Non-cyclic photophosphorylation
    • Cyclic photophosphorylation

C3, C4, and CAM Pathways

  • C3 plants are common (e.g., rice, wheat, and soybeans)
  • C4 plants have an alternative carbon fixation method for minimizing photorespiration, commonly seen in sugarcane and corn
  • CAM plants minimize photorespiration by opening stomata at night, for example pineapple and cacti.
  • Photorespiration occurs in C3 plants when stomata close.

Additional Notes

  • Different types of chlorophyll exist
  • Three compounds are formed during light reactions (NADPH, ATP, Oxygen)
  • C4 pathway has bundle sheath cells and mesophyll cells.
  • CAM pathway involves night storage of carbon dioxide
  • Chlorophyll degradation can result from osmotic stress.

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