Nucleic Acids: DNA and RNA Structure

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Questions and Answers

What is the primary function of nucleic acids within a cell?

  • Carrying genetic information (correct)
  • Catalyzing metabolic reactions
  • Structural support
  • Energy storage

Transcription is the process by which RNA is used to synthesize proteins.

False (B)

Name the process by which information stored in DNA is transferred to RNA.

transcription

The building blocks of nucleic acids are called ________.

<p>nucleotides</p>
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Which of the following is a component of a nucleotide?

<p>Sugar (C)</p>
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Uracil is a nitrogenous base found in DNA.

<p>False (B)</p>
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What type of bond links nucleotides together in a strand of DNA or RNA?

<p>phosphodiester bond</p>
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In the DNA double helix, adenine pairs with ________.

<p>thymine</p>
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Which structural description best describes the overall shape of a DNA molecule?

<p>Double helix (B)</p>
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The two strands of DNA in a double helix run parallel to each other.

<p>False (B)</p>
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What enzyme is responsible for unwinding the DNA double helix during replication?

<p>helicase</p>
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The strand of DNA that is synthesized continuously during replication is known as the ________ strand.

<p>leading</p>
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Which of the following enzymes is primarily responsible for adding nucleotides to the growing DNA strand during replication?

<p>DNA polymerase (C)</p>
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Primase is required to initiate DNA replication on both the leading and lagging strands.

<p>True (A)</p>
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What are the short, discontinuous DNA fragments produced on the lagging strand during DNA replication called?

<p>Okazaki fragments</p>
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The enzyme that joins Okazaki fragments together on the lagging strand is called ________.

<p>ligase</p>
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During DNA replication, what is the role of single-strand binding proteins?

<p>To prevent the separated DNA strands from re-annealing (B)</p>
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DNA replication always starts at a single origin of replication in eukaryotic cells.

<p>False (B)</p>
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In transcription, what serves as the template for mRNA synthesis?

<p>DNA</p>
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In mRNA, a sequence of three nucleotides that codes for an amino acid is called a ________.

<p>codon</p>
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What is the function of tRNA in translation?

<p>To transport amino acids to the ribosome (B)</p>
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Ribosomes are only found attached to the endoplasmic reticulum.

<p>False (B)</p>
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Name the process by which the genetic code in mRNA is used to synthesize a protein.

<p>translation</p>
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During translation, mRNA is read in a ________ to ________ direction.

<p>5', 3'</p>
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Match the following enzymes with their function in DNA replication:

<p>Helicase = Unwinds the DNA double helix DNA Polymerase = Adds nucleotides to the growing DNA strand Primase = Synthesizes RNA primers Ligase = Joins Okazaki fragments</p>
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Flashcards

What is DNA?

Deoxyribonucleic acid (DNA) is a macromolecule that provides storage and transmission of genetic information in the form of a genetic code consisting of a sequence of nucleotides.

What is transcription?

The process where information stored in DNA is copied into RNA.

What is translation?

The process where the information in RNA is used to synthesize proteins.

What are nucleotides?

Subunits that make up nucleic acids. They consist of a sugar, a phosphate group, and a nitrogenous base.

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What are nitrogenous bases?

The nitrogenous bases in DNA and RNA that include adenine (A), guanine (G), cytosine (C), thymine (T) in DNA, and uracil (U) in RNA.

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What is a phosphodiester bond?

A type of covalent bond that links nucleotides together in DNA and RNA between the sugar and phosphate groups.

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What is Helicase?

Enzyme that unwinds the double helix structure of DNA, breaking hydrogen bonds between base pairs.

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What is DNA polymerase?

One of the main enzymes involved in DNA replication, It is responsible for adding nucleotides to the growing DNA strand.

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What is a primer?

A short strand of RNA or DNA that serves as a starting point for DNA synthesis.

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What is Primase?

Enzyme that synthesizes short RNA sequences called primers, which serve as a starting point for DNA synthesis.

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What is Ligase?

An enzyme that catalyzes the joining of DNA strands together by forming phosphodiester bonds.

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Study Notes

  • Nucleic acids include DNA and RNA.
  • They act as carriers of genetic information.
  • DNA acts as a library of stored information.
  • Information stored in DNA is transcribed into RNA through a process called transcription.
  • Information in RNA is used to synthesize proteins in Translation.

DNA Structure

  • DNA is composed of a long chain of subunits called nucleotides.
  • Nitrogenous groups in nucleotides in DNA and RNA are either purines or pyrimidines.
  • In RNA, Uracil is used instead of Thymine.
  • Nucleotides pair up with hydrogen bonds to form base pairs.
  • Thymine (in DNA) or Uracil (in RNA) pair up with Adenine with 2 hydrogen bonds.
  • Cytosine pairs up with Guanine with 3 hydrogen bonds.
  • Nucleotides can be linked by phosphodiester bonds at carbons 3 and 5.
  • DNA or Deoxyribonucleic acid is a macromolecule that provides storage and transmission of genetic information.
  • This information is implemented for the development and functioning of living organisms.
  • A DNA molecule stores biological information in the form of a genetic code of nucleotides.
  • DNA structure has two strands that form a double helix.
  • Antiparallel strands of DNA are twisted to form a double helix.

DNA Replication

  • DNA Replication: DNA unzips, creating leading and lagging strands.
  • Helicase creates this split.

Enzymes in DNA Replication

  • Helicase unzips DNA.
  • DNA Polymerase acts as a builder during replication.
  • Primase is the initializer.
  • Ligase acts as a gluer.

Transcription

  • Transcription of DNA creates mRNA.
  • Translation of mRNA translated at ribosomes with the help of tRNA.
  • Ribosomes are found loose in the cytosol and at the rough endoplasmic reticulum.
  • Genetic information is stored as a three-letter code or codon.

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