Podcast
Questions and Answers
Why is it important to flame the mouth of a culture tube before and after inserting an inoculation loop?
Why is it important to flame the mouth of a culture tube before and after inserting an inoculation loop?
- To ensure the bacteria are evenly distributed within the media.
- To create a sterile environment that prevents contaminants from entering the tube. (correct)
- To quickly evaporate any condensation that may have formed inside the tube.
- To cool the glass so it doesn't shatter when the lid is replaced.
In which scenario is it essential to allow the inoculation tool to cool after sterilization but before collecting a sample?
In which scenario is it essential to allow the inoculation tool to cool after sterilization but before collecting a sample?
- When flaming the loop after inoculation is complete.
- Transferring bacteria from a Petri plate to a stab culture. (correct)
- Transferring bacteria from a liquid culture to liquid media.
- Transferring bacteria from a liquid culture to a Petri dish.
When using a Bunsen burner, which flame indicates the highest temperature and is most suitable for sterilizing inoculation tools?
When using a Bunsen burner, which flame indicates the highest temperature and is most suitable for sterilizing inoculation tools?
- A medium blue flame.
- A pale orange flame with a yellow tip.
- A yellow and orange flame.
- A roaring blue flame with a clear blue cone. (correct)
A researcher is struggling to ignite their Bunsen burner. What is the most likely cause?
A researcher is struggling to ignite their Bunsen burner. What is the most likely cause?
Why rotate the Petri dish when spreading bacteria transferred from a liquid culture?
Why rotate the Petri dish when spreading bacteria transferred from a liquid culture?
A student accidentally sets a sterilized inoculation loop down on the lab bench. Which action should they take next?
A student accidentally sets a sterilized inoculation loop down on the lab bench. Which action should they take next?
A scientist flamed the loop, transferred bacteria from a liquid culture to sterile liquid media, flamed the mouth, and replaced the lid. What is the next step?
A scientist flamed the loop, transferred bacteria from a liquid culture to sterile liquid media, flamed the mouth, and replaced the lid. What is the next step?
In the procedure for creating a stab culture, why is it important to perform the 'stab' three times?
In the procedure for creating a stab culture, why is it important to perform the 'stab' three times?
During aseptic transfer of a bacterial culture to a Petri dish, a student finds that the lid of the dish keeps getting in the way. Which of the following is the correct technique for handling the lid?
During aseptic transfer of a bacterial culture to a Petri dish, a student finds that the lid of the dish keeps getting in the way. Which of the following is the correct technique for handling the lid?
A researcher is preparing to transfer bacteria and notices condensation in the culture tubes. What is the best course of action?
A researcher is preparing to transfer bacteria and notices condensation in the culture tubes. What is the best course of action?
Flashcards
Aseptic Technique
Aseptic Technique
A technique used to transfer a pure bacterial culture into a new medium without contamination.
Inoculation Loops/Needles
Inoculation Loops/Needles
Common tools, sterilized by heat, for transferring bacteria.
Bunsen Burner
Bunsen Burner
A gas burner that produces a sterile field and sterilizes tools.
Hottest Flame Tip
Hottest Flame Tip
Signup and view all the flashcards
Sterilizing Tools
Sterilizing Tools
Signup and view all the flashcards
Transferring Bacteria to Petri Dish
Transferring Bacteria to Petri Dish
Signup and view all the flashcards
Transferring Bacteria to Liquid Media
Transferring Bacteria to Liquid Media
Signup and view all the flashcards
Transferring Bacteria to Stab Culture
Transferring Bacteria to Stab Culture
Signup and view all the flashcards
Study Notes
- Aseptic technique enables transfer of a bacterial monoculture into a fresh medium without introducing contaminating microorganisms.
- Inoculation loops and needles are commonly used tools to transfer bacteria.
- Knowing how to use a Bunsen burner is essential for aseptic technique.
Lighting a Bunsen Burner
- Close the valve at the base by rotating the collar to cover the vents.
- Slowly turn on the gas.
- Ignite the burner once gas is heard.
- Adjust the flame height by controlling the gas flow.
Adjusting Flame Temperature
- Control oxygen flow by rotating the collar.
- Coolest flame: yellow and orange
- Medium flame: blue
- Hottest flame: roaring blue with a clear blue cone; the tip of the cone is the hottest part.
Sterilizing Inoculation Tools
- Pass tools through the hottest part of the flame.
- Sterilized tools should not be set down on any surface.
Transferring Bacteria from Liquid Culture to Petri Dish
- Flame the loop.
- Remove the lid of the liquid culture container.
- Pass the mouth of the container through the flame.
- Dip the loop into the liquid culture.
- Flame the mouth of the container again before replacing the lid.
- Transfer bacteria to the Petri plate.
- Lift the plate lid and hold it as a shield.
- Spread bacteria evenly across the plate, rotating a quarter turn between spreads.
Transferring Bacteria from Liquid Culture to Liquid Media
- Sterilize an inoculation loop.
- Remove the lid from the liquid culture container and flame the mouth.
- Dip the loop into the liquid culture, then flame the mouth again before replacing the lid.
- Take the tube of liquid media and flame the mouth.
- Dip the loop with bacteria into the tube.
- Flame the mouth of the tube before replacing the lid.
- Flame the loop to sterilize.
Transferring Bacteria from Petri Plate to Stab Culture
- Sterilize an inoculation needle by passing it through a flame.
- Allow the needle to cool; test on a clear area of the agar plate, holding the lid as a shield.
- Gently rub the needle over a single colony.
- Transfer bacteria to a culture tube filled with nutrient agar.
- Remove the tube top and insert the needle, making three deep stabs.
- Replace the lid and sterilize the needle.
Transferring Bacteria from Petri Plate to Liquid Media
- Sterilize an inoculation loop by passing it through a flame.
- Allow the loop to cool; test on a clear area of the agar plate, holding the lid as a shield.
- Gently rub the loop over a single colony.
- Take the tube of liquid media, remove the lid, and flame the mouth.
- Dip the loop with bacteria into the tube.
- Flame the mouth of the container before replacing the lid.
- Sterilize the inoculation loop to complete the process.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Learn aseptic techniques for transferring bacterial cultures without contamination. Master Bunsen burner usage, flame sterilization of inoculation tools, and transferring bacteria from liquid culture to Petri dishes. Proper aseptic technique ensures pure culture maintenance.
